K+-stimulated sugar uptake in skeletal muscle: role of cytoplasmic Ca2+.
K+-stimulated sugar uptake in skeletal muscle: role of cytoplasmic Ca2+.
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K 刺激骨骼肌中的糖摄取：细胞质 Ca2 的作用。
DOI：
10.1152/ajpcell.1983.245.1.c125
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Erlij,D
中科院分区：
文献类型：
--
作者：
Valant,P;Erlij,D
我们比较了胰岛素与升高的外部 K+ 浓度 ([K+]o) 对青蛙骨骼肌糖摄取和蛋白质合成的影响。当 [K+]o 在 0.5 至 15 mM 之间时，对摄取没有影响；然而，当使用 20 mM 时，观察到显着增加。 [K+]o 的进一步增加引起更大的吸收刺激。洗涤高 [K+]o 后刺激持续 2.5 小时。细胞松弛素 B 显着抑制了胰岛素和高 K+ 引起的刺激。丹曲林几乎消除了对高 K+ 的反应，而它对静息糖摄取以及胰岛素或肾上腺素引起的刺激只有轻微影响。这些结果表明，虽然胰岛素和高 K+ 都能激活糖转运的细胞松弛素敏感转运系统，但每种药物必须通过不同的途径发挥作用，因为只有高 K+ 的作用对丹曲林敏感。丹曲林的作用表明，高 K+ 引起的糖转运增强是由于细胞质 Ca2+ 的增加。与胰岛素相比，高 K+ 不会改变蛋白质合成速率。
We have compared the effects of insulin with those of elevated external K+ concentration ([K+]o) on sugar uptake and protein synthesis by frog skeletal muscle. When [K+]o was between 0.5 and 15 mM there were no effects on uptake; however, when 20 mM was used a significant increase was observed. Further increases in [K+]o caused larger stimulations of uptake. The stimulation persisted for 2.5 h after washing the high [K+]o. The stimulations caused by both insulin and high K+ were markedly inhibited by cytochalasin B. Dantrolene nearly abolished the response to high K+, whereas it had only minor effects on the resting sugar uptake and on the stimulations caused by either insulin or epinephrine. These results suggest that while both insulin and high K+ activate the cytochalasin-sensitive transport system of sugar transport, each agent must act through a different pathway, because only the effects of high K+ were dantrolene sensitive. The effect of dantrolene suggests that the enhancement of sugar transport caused by high K+ is due to an increase of cytoplasmic Ca2+. In contrast to insulin, high K+ did not modify the rate of protein synthesis.