Update on the major clonal types of methicillin-resistant Staphylococcus aureus in the Czech Republic
Update on the major clonal types of methicillin-resistant Staphylococcus aureus in the Czech Republic
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DOI：
10.1128/jcm.41.11.4998-5005.2003
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发表时间：
2003-11-01
影响因子：
9.4
通讯作者：
de Lencastre, H
中科院分区：
文献类型：
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作者：
Melter, O;de Sousa, MA;de Lencastre, H
本研究的目的是分析2000-2002年间捷克21家医院分离的耐甲氧西林金黄色葡萄球菌(MRSA)的分子特征，并与1996-1997年间的结果进行比较。对菌株进行SmaI酶切的脉冲场凝胶电泳(PFGE)和16S-23S DNA探针杂交的HindIII酶切谱的核糖体分型。对临床上最重要的大环内酯类(ermA、ermB、ermC和msrA)和氨基糖苷类(ap3‘、ant4’和aac6‘-ap2“)耐药基因的流行率也进行了评估。对每种克隆型的代表菌株进行了多位点序列分型和多重聚合酶链式反应分析，该方法能够识别细菌携带的葡萄球菌盒式染色体mEC的结构类型。我们的结果记录了巴西克隆(ST239，SCCmec IIIA，PFGE B型，核糖体H1型)被我们命名为“捷克克隆”(ST239，SCCmec IIIA，PFGE F型，核型H6)的新克隆的置换，以及伊比利亚克隆(ST247，SCCmec IA，PFGE A型，核型H2型)的维护。此外，我们还发现氨基糖苷类耐药基因的分布与MRSA克隆型之间存在相关性。
The purpose of our study was the molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in 21 hospitals in the Czech Republic in the period 2000-2002 and comparison with previous results from 1996-1997. Strains were analyzed by pulsed-field gel electrophoresis (PFGE) of SmaI digests and ribotyping of HindIII digests hybridized with a 16S-23S DNA probe. The prevalence of the most clinically important macrolide (ermA, ermB, ermC, and msrA) and aminoglycoside (aph3', ant4', and aac6'-aph2") resistance genes was evaluated as well. Selected isolates representative of each clonal type were analyzed by multilocus sequence typing and by a multiplex PCR method capable of identifying the structural type of the staphylococcal cassette chromosome mec (SCCmec) carried by the bacteria. Our results document the displacement of the Brazilian clone (ST239, SCCmec type IIIA, PFGE type B, ribotype H1) by a new clone that we named "Czech clone" (ST239, SCCmec type IIIA, PFGE type F, ribotype H6) and the maintenance of the Iberian clone (ST247, SCCmec type IA, PFGE type A, ribotype H2) exclusively in one hospital in the Czech Republic. In addition, we found a correlation between the distribution of aminoglycoside resistance genes and MRSA clonal types.