ANALYSIS OF 17D YELLOW-FEVER VIRUS ENVELOPE PROTEIN EPITOPES USING MONOCLONAL-ANTIBODIES
ANALYSIS OF 17D YELLOW-FEVER VIRUS ENVELOPE PROTEIN EPITOPES USING MONOCLONAL-ANTIBODIES
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DOI：
10.1099/0022-1317-65-10-1637
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发表时间：
1984-01-01
影响因子：
3.8
通讯作者：
BRANDRISS, MW
中科院分区：
文献类型：
--
作者：
SCHLESINGER, JJ;WALSH, EE;BRANDRISS, MW
Sixteen monoclonal antibodies that reacted with the envelope glycoprotein (E) of 17D vaccine strain yellow fever virus (17D YF), including 2 antibodies produced against dengue 2 virus, were used in a solid phase competitive binding assay (CBA) to define spatial relationships among antigenic determinants on 17D, YF E. The antibodies showed YF strain, type or flavivirus group specificities and 9 epitopes were identified on 17D YF E by patterns of neutralization, hemagglutination inhibition and competition of antibody binding. Epitopes defined by neutralizing antibodies with strain and type specificities appeared spatially distant but competition between type-specific neutralizing antibodies and some non-neutralizing antibodies against type and group determinants suggested close proximity among epitopes in these regions. Despite competition between some neutralizing and non-neutralizing monoclonal antibodies in CBA, plaque assays revealed no interference with neutralization by non-neutralizing antibody.
