Fluorescence detection and identification of nitro derivatives of polynuclear aromatic hydrocarbons by on-column catalytic reduction to aromatic amines
Fluorescence detection and identification of nitro derivatives of polynuclear aromatic hydrocarbons by on-column catalytic reduction to aromatic amines
复制标题
柱上催化还原芳香胺荧光检测鉴定多核芳烃硝基衍生物
DOI：
10.1021/ac00121a050
复制
发表时间：
1986
影响因子：
7.4
通讯作者：
J. E. Sigsby
中科院分区：
文献类型：
--
作者：
S. Tejada;R. Zweidinger;J. E. Sigsby
痕量多环芳烃的硝基衍生物(Nitro-PAH)在铂和Rh包覆的5-Mu·m氧化铝催化剂柱上被有效地还原为伯芳胺。还原是在以甲醇/水为流动相的反相高效液相系统中完成的。还原是经典的催化；即，不消耗铂/Rh催化剂。荧光测量允许在低皮克水平下检测到一些硝基多环芳烃作为芳香胺。给出了19种芳香胺的荧光检测的激发波长和发射波长及其荧光光谱。有些光谱表现出独特的特征，这对确认身份很有用。建立了一种基于多维高效液相色谱原理的分析系统，用于柴油机排气颗粒物萃取物等复杂有机基质中硝基多环芳烃的常规分析。
Nitro derivatives of polynuclear aromatic hydrocarbons (nitro-PAH) at trace-level concentrations were efficiently reduced to primary aromatic amines on a catalyst column packed with 5-..mu..m alumina that was coated with platinum and rhodium (Pt/Rh). The reduction was accomplished in a reversed-phase HPLC system using methanol/water for the mobile phase. Reduction is classically catalytic; i.e., the Pt/Rh catalyst is not consumed. Fluorescence measurements permitted detection of some nitro-PAH as aromatic amines at low picogram levels. Excitation and emission wavelengths for fluorescence detection of 19 aromatic amines are given along with their fluorescence spectra. Some spectra exhibit unique features that are useful for confirming identification. An analytical system based on multidimensional HPLC principles was developed for routine analysis of specific nitro-PAH in complex organic matrices such as extracts of diesel exhaust particulates with minimal sample preparation.