Screening, determination and confirmation of chloramphenicol in seafood, meat and honey using ELISA, HPLC-UVD, GC-ECD, GC-MS-EI-SIM and GCMS-NCI-SIM methods
Screening, determination and confirmation of chloramphenicol in seafood, meat and honey using ELISA, HPLC-UVD, GC-ECD, GC-MS-EI-SIM and GCMS-NCI-SIM methods
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DOI：
10.1016/j.aca.2004.12.027
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发表时间：
2005-04-11
影响因子：
6.2
通讯作者：
Jiang, HL
中科院分区：
文献类型：
--
作者：
Shen, HY;Jiang, HL
酶联免疫吸附法（ELISA）筛选、测定和确认海鲜、肉类、蜂蜜等10种基质中氯霉素（CAP）的研究在电子电离模式（El）和负离子化学电离模式（NCI）下，采用选择离子监测（SIM）采集方法（GC-MS- ei -SIM和GCMS-NCI-SIM）进行了高效液相色谱(HPLC) -紫外检测器（HPLC- uvd）和气相色谱(GC-ECD) -质谱检测器（GC-MS）联用。建立了氯霉素（CAP）的定性和定量检测方法。对萃取、净化、衍生和分析程序进行了优化。采用酶联免疫吸附法（ELISA）筛选，HPLC、GC和GC- ms对可疑样品中CAP残留浓度进行测定和确认。ELISA程序在样品的水萃取物上进行。可疑样品经磷酸缓冲液(PBS, pH = 6.88)/乙酸乙酯萃取，己烷脱脂后，采用HPLC或GC-ECD、GC-MS-El、GC-MS-NCI法测定和确认。用于GC分析的样品，用LC-Si和LC-C18筒进行固相萃取净化，用衍生化剂衍生成挥发性衍生物。这些技术可检测出0.1 ~ 10 μ g/kg的氯霉素残留量。加样回收率为75 ~ 120%，rsd值为5.4 ~ 8.1%。(c) 2004 Elsevier B.V.版权所有
Studies on screening, determination and confirmation of chloramphenicol (CAP) in 10 kinds of matrices, including seafood, meat, honey, etc. by enzyme-linked immunosorbent assay (ELISA), high-performance liquid chromatography (HPLC) with UV detector (HPLC-UVD) and gas chromatography in combination with electronic capture detector (GC-ECD) and mass spectrometry detector (GC-MS) in both electronic ionization mode (El) and the negative ion chemical ionization mode (NCI) with selected-ion monitoring (SIM) acquisition method (GC-MS-EI-SIM and GCMS-NCI-SIM) have been carried out. Methods have been developed for both qualitative and quantitative detection of chloramphenicol (CAP). Extraction, clean-up, derivatization and analysis procedure have been optimized. The ELISA was carried out for screening, HPLC, GC and GC-MS were applied to determine and confirm CAP residue concentration in suspect samples. The ELISA procedure was carried out on an aqueous extract of the samples. Determination and confirmation of suspect samples were performed after extraction with phosphoric buffer solution (PBS, pH = 6.88)/ethyl acetate, defatting with hexane, analyzed by HPLC or GC-ECD, GC-MS-El, GC-MS-NCI method. Samples for GC analysis, were further clean-up with solid-phase extraction using LC-Si and LC-C18 cartridges and derivatized to form volatile derivatives by derivatization agent. These techniques are able to detect chloramphenicol residues at the level of 0.1-10 mu g/kg. Overall recoveries were 75-120% with R.S.D. values at 5.4-8.1%. (c) 2004 Elsevier B.V. All rights reserved.