Expression and SNP association analysis of porcine FBXL4 gene
Expression and SNP association analysis of porcine FBXL4 gene
复制标题
猪FBXL4基因的表达及SNP关联分析
DOI：
10.1007/s11033-009-9825-7
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发表时间：
2010-01-01
影响因子：
2.8
通讯作者：
Peng, K. M.
中科院分区：
文献类型：
--
作者：
Li, Y.;Yang, S. L.;Peng, K. M.
FBXL 4基因的产物是E3连接酶的一种，属于F-BOX蛋白中最大的FBLs亚家族成员，通过特定的蛋白质相互作用域识别底物。为了研究其功能，对其进行了多态性分析和关联分析。首次克隆了猪FBXL 4基因的部分cDNA，全长2384 bp，推导的蛋白质在第277 ~ 332位氨基酸处含有一个保守的F-BOX结构域。系统进化树显示，猪FBXL 4与牛FBXL 4的亲缘关系最近。定量RT-PCR分析表明，猪FBXL 4基因在10个组织中的表达量波动较大。Tail基因分型结果表明，在引进长白猪、小型贵州猪和五指山猪中TT基因型占优势，而在小型莱芜猪中CC基因型多于其他2种基因型。而在BsaJI的另一个基因型分析中，两个中国小型猪品种和引进长白猪品种中CC基因型明显多于其他基因型。与免疫性状和血液指标的关联分析表明，SNP Tail与0日龄仔猪淋巴细胞百分率（P = 0.0166）和伪狂犬病疫苗抗体水平（P = 0.0001）显著相关。同时，SNP BsaJI与个体32日龄淋巴细胞百分率（P = 0.0351）、新生仔猪0日龄中性粒细胞百分率（P = 0.0005）、淋巴细胞绝对计数（P = 0.0458）和混合细胞（P = 0.0010）显著相关。
As a kind of E3 ligase, the product of FBXL4 gene belongs to a member of FBLs which is the biggest eukaryotic subfamily of F-BOX proteins, it can recognize some substrate through particular protein-protein interaction domains. To investigate its functions, the polymorphism and association analysis was analyzed. The partial cDNA of porcine FBXL4 with 2384 bp long was first cloned; the deduced protein comprises a conserved F-BOX domain at position from the 277th to 332nd amino acid. The phylogenetic tree indicated porcine FBXL4 has the closest genetic relationship with bovine FBXL4 than other selected animal species. Ten tissue expression level of porcine FBXL4 mRNA fluctuated remarkably in a large range by quantitative RT-PCR analysis. For two identified SNPs, the genotyping analysis of Tail showed TT genotype owned dominance in introduced Landrace pig and miniature Guizhou and Wuzhishan breeds, but CC genotype was more than two other genotypes in miniature Laiwu breed. While in another genotyping analysis of BsaJI, CC genotype was obviously more than other genotypes in two kinds of Chinese miniature pig breeds and introduced Landrace pig breeds. Furthermore, the association analysis with immune traits and blood parameters revealed that SNP Tail was significantly associated with the lymphocyte percentage (P = 0.0166) and the antibody levels for pseudorabies virus vaccination (P = 0.0001) of neonate piglets at 0 day. Meanwhile, SNP BsaJI was significantly associated with lymphocyte percentage of individuals at 32 days (P = 0.0351), neutrophil percentage (P = 0.0005), the absolute lymphocyte count (P = 0.0458), and the mixed cells (P = 0.0010) of neonate piglets at 0 day.