EMT tumorigenesis in the mouse mammary gland
EMT tumorigenesis in the mouse mammary gland
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DOI：
10.1038/labinvest.3700683
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发表时间：
2007-12-01
影响因子：
5
通讯作者：
Cardiff, Robert D.
中科院分区：
文献类型：
--
作者：
Damonte, Patrizia;Gregg, Jeffrey P.;Cardiff, Robert D.
术语EMT（上皮-间质转化）用于许多环境中。该术语用于描述在胚胎发育和损伤后组织重建期间促进细胞重新定位和重新部署的机制。最近，EMT也被应用于恶性进展的潜在机制，并已成为肿瘤的特定诊断类别。在小鼠中，大多数“EMT”肿瘤具有梭形细胞表型。EMT的定义是有争议的，因为梭形细胞肿瘤在人类中并不常见，特别是在人类乳腺癌中。小鼠乳腺梭形细胞瘤已被观察多年，它们通常被分类为肉瘤或癌肉瘤。基因工程小鼠发生乳腺梭形细胞肿瘤，似乎出现在上皮细胞中并经历EMT。为了更好地了解这些梭形细胞肿瘤在进展和转移中的起源和演变，研究了来自加州大学戴维斯分校突变小鼠病理学实验室档案的7个梭形细胞肿瘤队列。这项研究提供了EMT的实验和免疫组化证据，表明肿瘤梭形细胞的上皮和间充质双重染色识别了一些，但不是所有的小鼠EMT型肿瘤。这表明潜在的EMT肿瘤最好被指定为EMT表型肿瘤。
The term EMT (epithelial-mesenchymal transition) is used in many settings. This term is used to describe the mechanisms facilitating cellular repositioning and redeployment during embryonic development and tissue reconstruction after injury. Recently, EMT has also been applied to potential mechanisms for malignant progression and has appeared as a specific diagnostic category of tumors. In mice, most 'EMT' tumors have a spindle cell phenotype. The definition of EMT is controversial because spindle cell tumors are not common in humans, especially in human breast cancers. Spindle cell tumors of the mouse mammary gland have been observed for many years where they are usually classified as sarcomas or carcinosarcomas. Genetically engineered mice develop mammary spindle cell tumors that appear to arise in the epithelium and undergo EMT. To better understand the origin and evolution of these spindle cell tumors in progression and metastases, seven cohorts of spindle cell tumors from the archives of the University of California, Davis Mutant Mouse Pathology Laboratory were studied. This study provides experimental and immunohistochemical evidence of EMT showing that dual epithelial and mesenchymal staining of tumor spindle cells identifies some, but not all, EMT-type tumors in the mouse. This suggests that potential EMT tumors are best designated EMT-phenotype tumors.