Endosialin (TEM1, CD248) is a marker of stromal fibroblasts and is not selectively expressed on tumour endothelium
Endosialin (TEM1, CD248) is a marker of stromal fibroblasts and is not selectively expressed on tumour endothelium
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DOI：
10.1016/j.febslet.2005.03.071
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发表时间：
2005-05-09
期刊：
影响因子：
3.5
通讯作者：
Isacke, CM
中科院分区：
文献类型：
--
作者：
MacFadyen, JR;Haworth, O;Isacke, CM
成纤维细胞是一种多样的细胞类型，并显示出清晰的地形分化和位置记忆。在成纤维细胞特异性标记物的筛选中，我们已经表征了四种单克隆抗体;内皮唾液素（TEM 1/CD 248）。以前的研究报道，内皮唾液酸是一种肿瘤内皮标志物，并定位于细胞内。我们最终证明，内皮素是一种细胞表面糖蛋白，主要由成纤维细胞和与肿瘤血管相关的周细胞亚群表达，但不是由肿瘤内皮细胞。这些新的抗体将有助于分离和分类的成纤维细胞和周细胞谱系，以及进一步的功能分析的内皮唾液酸。(c)2005年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Fibroblasts are a diverse cell type and display clear topographic differentiation and positional memory. In a screen for fibroblast specific markers we have characterized four monoclonal antibodies; to endosialin (TEM1/CD248). Previous studies have reported that endosialin is a tumour endothelium marker and is localized intracellularly. We demonstrate conclusively that endosialin is a cell surface glycoprotein and is predominantly expressed by fibroblasts and a subset of pericytes associated with tumour vessels but not by tumour endothelium. These novel antibodies will facilitate the isolation and classification of fibroblast and pericyte lineages as well as the further functional analysis of endosialin. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.