Studies on the development of mouse embryos in vitro. IV. Interaction of energy sources.
Studies on the development of mouse embryos in vitro. IV. Interaction of energy sources.
复制标题
DOI：
10.1530/jrf.0.0100227
复制
发表时间：
1964-12
期刊：
影响因子：
--
通讯作者：
R. Brinster
中科院分区：
文献类型：
--
作者：
R. Brinster
使用统计实验设计检查了一些可能的2细胞小鼠卵子体外发育的能量来源的相互作用。这些实验表明，当葡萄糖与丙酮酸的最佳浓度一起使用时，对发育没有有益的影响。丙酮酸和草酰乙酸的最佳浓度在一起使用时的反应明显低于单独使用时的反应。结果表明，2细胞小鼠卵细胞发育成囊胚的最佳培养基为2.5 ~ 5.5 × 10−4 m -丙酮酸+ 2.5 ~ 5.5 × 10−2 m -乳酸。
The interactions of a number of possible energy sources for in-vitro development of 2-cell mouse ova were examined using statistical experimental designs. These experiments indicated that glucose has no beneficial effect on development when employed with the optimum concentration of pyruvate. Optimum concentrations of pyruvate and oxaloacetate when employed together resulted in a significantly lower response than when either compound was employed alone. It was found that the best medium for the development of 2-cell mouse ova into blastocysts contained 2·5 to 5·0 × 10−4 M-pyruvate + 2·5 to 5·0 × 10−2 M-lactate.