NMB, A NOVEL GENE, IS EXPRESSED IN LOW-METASTATIC HUMAN-MELANOMA CELL-LINES AND XENOGRAFTS
NMB, A NOVEL GENE, IS EXPRESSED IN LOW-METASTATIC HUMAN-MELANOMA CELL-LINES AND XENOGRAFTS
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DOI：
10.1002/ijc.2910600111
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发表时间：
1995-01-03
影响因子：
6.4
通讯作者：
BLOEMERS, HPJ
中科院分区：
文献类型：
--
作者：
WETERMAN, MAJ;AJUBI, N;BLOEMERS, HPJ
从消减cDNA文库中，我们分离出几个cDNA克隆，这些克隆在高转移性和低转移性人黑色素瘤细胞系之间表现出差异表达。命名为nmb的一个克隆在低转移细胞系中显示优先表达，并被选择用于进一步表征。序列分析表明，该克隆代表了一个新的基因，编码一个推定的跨膜糖蛋白，显示出最高的同源性pMEL 17，黑素细胞特异性蛋白质的前体。nmb RNA表达在大多数测试的肿瘤细胞系中不存在，并且不限于黑素细胞谱系。将部分nmb cDNA转染到高转移性黑色素瘤细胞系（BLM）中，在3个转染子中的2个中，导致皮下肿瘤生长较慢，在3个转染子中的1个中，导致裸鼠自发转移的可能性降低。(C)1995 Wiley-Liss，Inc.
From a subtractive cDNA library, we isolated several cDNA clones which showed differential expression between highly and lowly metastatic human melanoma cell lines. One clone, designated nmb, showed preferential expression in the low-metastatic cell lines and was chosen for further characterization. Sequence analysis revealed that this clone represents a novel gene, encoding a putative transmembrane glycoprotein which showed the highest homology to the precursor of pMEL17, a melanocyte-specific protein. nmb RNA expression was absent in most tumor-cell lines tested and not restricted to the melanocytic lineage. Transfection of a partial nmb cDNA into a highly metastatic melanoma cell line (BLM) resulted, in 2 of 3 transfectants, in slower subcutaneous tumor growth and, in 1 of 3 transfectants, in reduction of the potential for spontaneous metastasis in nude mice. (C) 1995 Wiley-Liss, Inc.