Cell movement patterns during gastrulation in the chick are controlled by chemotaxis mediated by positive and negative FGF4 and FGF8
Cell movement patterns during gastrulation in the chick are controlled by chemotaxis mediated by positive and negative FGF4 and FGF8
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DOI：
10.1016/s1534-5807(02)00256-3
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发表时间：
2002-09-01
影响因子：
11.8
通讯作者：
Weijer, CJ
中科院分区：
文献类型：
--
作者：
Yang, XS;Dormann, D;Weijer, CJ
在羊膜动物原肠胚形成过程中，外胚层细胞通过原条进入并迁移出去，形成内胚层、中胚层和胚外结构。在这里，我们使用 GFP 标记的条纹细胞运动的体内成像来分析原始条纹不同前后位置出现的细胞的详细运动轨迹。从条纹的不同前后位置出现的细胞显示出特征性的细胞迁移模式，以响应来自邻近组织的引导信号。条纹细胞被 FGF4 来源吸引，并被 FGF8 来源排斥。观察到的前条纹细胞的运动模式可以解释为 FGF8 介导的细胞化学排斥远离条纹，随后对形成的脊索产生的 FGF4 信号进行化学吸引。
During gastrulation in amniotes, epiblast cells ingress through the primitive streak and migrate away to form endodermal, mesodermal, and extraembryonic structures. Here we analyze the detailed movement trajectories of cells emerging at different anterior-posterior positions from the primitive streak, using in vivo imaging of the movement of GFP-tagged streak cells. Cells emerging at different anterior-posterior positions from the streak show characteristic cell migration patterns, in response to guidance signals from neighboring tissues. Streak cells are attracted by sources of FGF4 and repelled by sources of FGF8. The observed movement patterns of anterior streak cells can be explained by an FGF8-mediated chemorepulsion of cells away from the streak followed by chemoattraction toward an FGF4 signal produced by the forming notochord.