Identification of optimal molecular size of modified Aloe polysaccharides with maximum immunomodulatory activity
Identification of optimal molecular size of modified Aloe polysaccharides with maximum immunomodulatory activity
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DOI：
10.1016/j.intimp.2004.09.031
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发表时间：
2005-02-01
影响因子：
5.6
通讯作者：
Lee, CK
中科院分区：
文献类型：
--
作者：
Im, SA;Oh, ST;Lee, CK
已知从芦荟种的凝胶中分离的多糖具有多种生物活性。包括免疫调节和抗肿瘤活性。以芦荟凝胶为原料，经纤维素酶部分酶解，制备了芦荟多糖（MAP）粗品（G2 E1），研究了MAP的分子大小与其免疫调节活性的关系。通过DEAE-Sephacel柱除去粗MAP中的蛋白质。然后将无蛋白MAP（G2 E1 D）进一步分离成三个级分。G2 EIDS 3分子量（MW大于或等于400 KDa）、G2 E1 DS 2（5 KDa小于或等于MW小于或等于5400 KDa）、G2 EIDS 1（MW小于或等于55 KDa）。用Sephacryl柱层析和超滤法测定MAP制剂对小鼠巨噬细胞的免疫调节活性。RAW 264.7细胞。移植肉瘤180细胞的ICR系小鼠的免疫组化结果显示，肉瘤180细胞对ICR系小鼠的免疫抑制作用较弱。我们发现，400和5 KDa之间的多糖表现出最有效的巨噬细胞活化活性，增加细胞因子的产生。一氧化氮释放表面分子的表达和吞噬活性。根据体外活性，在400和5 KDa之间的多糖在体内也表现出最强的抗肿瘤活性。(C)2004 Elsevier B.V保留所有权利。
Polysaccharides isolated from the gel of Aloe species have been known to have diverse biological activities. including immunomodulatory and antitumor activities. The molecular size-immunomodulatory activity relationship of modified Aloe polysaccharide (MAP) was examined in this study, Crude MAP (G2E1), was prepared from the gel of Aloe vera that was partially digested with cellulase. Proteins in crude MAP were removed by passage through a DEAE-Sephacel column. and then the protein-free MAP (G2E1D) was further separated into three fractions. G2EIDS3 molecular weight (MW greater than or equal to 400 KDa), G2E1DS2 (5 KDa less than or equal to MW less than or equal to 5400 KDa), G2EIDSI (MW less than or equal to 55 KDa). by Sephacryl column chromatography and ultrafiltration-Immunomodulatory activities of MAP preparations were examined on a mouse macrophage cell line. RAW 264.7 cells. and in ICR strain of mouse implanted with sarcoma 180 cells. We found that polysaccharides between 400 and 5 KDa exhibit the most potent macrophage-activating activity as determined by increased cytokine production. nitric oxide release. expression of surface molecules, and phagocytic activity. In accordance with the in vitro activity, polysaccharides between 400 and 5 KDa also exhibited the most potent antitumor activity in vivo. (C) 2004 Elsevier B.V All rights reserved.