Enzymatic recovery of polyester building blocks from polymer blends
Enzymatic recovery of polyester building blocks from polymer blends
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DOI：
10.1016/j.procbio.2017.01.004
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发表时间：
2017-08-01
影响因子：
4.4
通讯作者：
Guebitz, Georg M.
中科院分区：
文献类型：
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作者：
Gamerith, Caroline;Zartl, Barbara;Guebitz, Georg M.
在这项研究中，我们研究了来自 Thermobifida cellulosilytica (Thc_Cut1) 的角质酶水解不同聚合物共混物中聚对苯二甲酸乙二醇酯 (PET) 部分的能力。使用傅里叶变换红外光谱 (FT-IR) 和差示扫描量热法 (DSC) 测定各种材料（包括市售瓶子和包装）的成分。当与未事先分离的包装和瓶子中与聚乙烯 (PE) 或聚酰胺 (PA) 混合的 PET 一起孵育时，Thc_Cut1 选择性水解 PET 部分，释放对苯二甲酸 (TPA) 和对苯二甲酸单 (2-羟乙酯) (MHET)。与结晶度较高的纯 PET 相比，聚合物共混物的水解程度高达 9 倍。研究了温度、粒径、结晶度和产物抑制等各种参数对 Thc_Cut1 水解 PET 部分的影响。当孵育温度从40℃升高到60℃时，产物的释放量最多增加10倍。粒径越小，水解速率越高。有趣的是，瓶子中的半结晶 (24%) PET 比无定形 PET 薄膜 (12%) 中的粉末水解得更快。观察到对苯二甲酸双(2-羟乙基)酯(BHET)对Thc_Cutl对PET的水解有抑制作用。 (C) 2017 Elsevier Ltd. 保留所有权利。
In this study we investigated the ability of a cutinase from Thermobifida cellulosilytica (Thc_Cut1) to hydrolyze poly (ethylene terephthalate) (PET) moieties in different polymer blends. The composition of various materials including commercial available bottles and packaging was determined using Fourier Transform InfraRed spectroscopy (FT-IR) and Differential Scanning Calorimetry (DSC). When incubated with PET blended with polyethylene (PE) or polyamide (PA) from packaging and bottles without prior separation, Thc_Cut1 selectively hydrolyzed the PET moieties releasing terephthalic acid (TPA) and mono(2-hydroxyethyl) terephthalate (MHET). Polymer blends were hydrolyzed in an up to 9 times higher extent compared to higher crystalline pure PET. The influence of various parameters like temperature, particle size, crystallinity and product inhibition on hydrolysis of PET moieties by Thc_Cut1 was investigated. The amount of products released was up to 10 times higher when the incubation temperature was increased from 40 degrees C to 60 degrees C. The smaller the particle size the higher the hydrolysis rates were. Interestingly, semi-crystalline (24%) PET from bottles was hydrolyzed faster than powder from amorphous PET films (12%). An inhibitory effect of bis(2-hydroxyethyl) terephthalate (BHET) on hydrolysis of PET by Thc_Cutl was observed. (C) 2017 Elsevier Ltd. All rights reserved.