Long-term culture and immortalization of epithelial cells from normal adult human pancreatic ducts transfected by the E6E7 gene of human papilloma virus 16.
Long-term culture and immortalization of epithelial cells from normal adult human pancreatic ducts transfected by the E6E7 gene of human papilloma virus 16.
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发表时间：
1996-06
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通讯作者：
T. Furukawa;William P. Duguid;L. Rosenberg;J. Viallet;D. Galloway;M. Tsao
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作者：
T. Furukawa;William P. Duguid;L. Rosenberg;J. Viallet;D. Galloway;M. Tsao
胰腺癌是人类最致命的癌症之一。这些癌症中的大多数发生在胰管上皮。胰腺癌发病机制的研究在很大程度上依赖于动物模型。已描述了使用来自大鼠和仓鼠胰管的可增殖培养上皮细胞的胰腺癌发生的体外模型。然而，由于无法获得来源于正常人类胰腺的可增殖培养的导管上皮细胞，人类模型一直不存在。本文报道了一种通过感染含有人乳头瘤病毒16型E6和E7基因的逆转录病毒，长期培养正常人和良性成人胰腺来源的胰管上皮细胞的方法。其中一株细胞已经永生，并已连续传代20多代。它们的生长依赖于锚定，在裸鼠体内不会致癌。这些细胞系和建立它们的方法可能为体外研究导管上皮在人类胰腺疾病和癌症中所起的作用提供新的途径。
Pancreatic cancer is one of the most lethal cancers in humans. The majority of these cancers arise from the pancreatic duct epithelium. Research into the pathogenesis of pancreatic carcinoma has largely relied on animal models. In vitro models of pancreatic carcinogenesis using propagable cultured epithelial cells derived from the pancreatic ducts of rats and hamsters have been described. A human model, however, has been nonexistent due to the unavailability of propagable cultured duct epithelial cells derived from normal human pancreas. We report here a reproducible method for the long-term culture of pancreatic duct epithelial cells derived from normal and benign adult human pancreata by infection with a retrovirus containing the E6 and E7 genes of the human papilloma virus 16. One of these cell lines has become immortal and has propagated continuously for more than 20 passages. They remain anchorage dependent in their growth and nontumorigenic in nude mice. These cell lines and the methodology described here to establish them may provide new avenues for in vitro studies of the roles played by duct epithelium in human pancreatic diseases and cancers.