LEUKEMOGENESIS BY BOVINE LEUKEMIA-VIRUS - PROVIRAL DNA INTEGRATION AND LACK OF RNA EXPRESSION OF VIRAL LONG TERMINAL REPEAT AND 3' PROXIMATE CELLULAR SEQUENCES
LEUKEMOGENESIS BY BOVINE LEUKEMIA-VIRUS - PROVIRAL DNA INTEGRATION AND LACK OF RNA EXPRESSION OF VIRAL LONG TERMINAL REPEAT AND 3' PROXIMATE CELLULAR SEQUENCES
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DOI：
10.1073/pnas.79.8.2465
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
MARBAIX, G
中科院分区：
文献类型：
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作者：
KETTMANN, R;DESCHAMPS, J;MARBAIX, G
用限制性内切酶EcoRI酶切牛白血病病毒（BLV）诱导的17个牛淋巴样肿瘤的DNA。使用BLV基因组特异性放射性探针进行过滤杂交分析显示，所有肿瘤至少含有1种或部分1种前病毒。用Sac I消化这些原病毒表明，.apprx中出现了缺失。25%的病例和涉及的序列位于原病毒的5“一半。克隆的2种不同前病毒的3”端邻近细胞序列与其他16种肿瘤DNA的相应序列无同源性，表明BLV前病毒可以在广泛的基因组位点上生存。没有检测到病毒DNA的转录，包括长末端重复序列，这表明病毒基因表达不是维持肿瘤状态所必需的。没有观察到3''-proximate细胞序列的表达，这表明在所检查的病例中没有发生proximate下游促进。
The DNA from 17 bovine lymphoid tumors induced by bovine leukemia virus (BLV) was digested with the restriction endonuclease EcoRI. Filter hybridization analysis using radioactive probes specific for the BLV genome showed that all tumors contained at least 1 or a portion of 1 provirus. Digestion of these proviruses with Sac I demonstrated that deletions occurred in .apprx. 25% of the cases and involved sequences located in the 5'' half of the provirus. No sequence homology was observed between the cloned proximate cellular sequences flanking 2 different proviruses at their 3'' end and the corresponding sequences in 16 other tumor DNA, thus showing that a wide range of genomic sites could accommodate BLV proviruses. Transcription of viral DNA including long terminal repeated sequences was not detected, suggesting that viral gene expression is not required for maintenance of the tumor state. No expression of 3''-proximate cellular sequences was observed, indicating that no proximate downstream promotion took place in the cases examined.