Photothermal Excitation of Neurons Using MXene: Cellular Stress and Phototoxicity Evaluation.
Photothermal Excitation of Neurons Using MXene: Cellular Stress and Phototoxicity Evaluation.
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使用 MXene 对神经元进行光热激发：细胞应激和光毒性评估。
DOI：
10.1002/adhm.202302330
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发表时间：
2023
影响因子：
10
通讯作者：
Cohen-Karni,Tzahi
中科院分区：
文献类型：
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作者：
Wang,Yingqiao;Hartung,JaneE;Goad,Adam;Preisegger,MatíasA;Chacon,Benjamin;Gold,MichaelS;Gogotsi,Yury;Cohen-Karni,Tzahi
理解单个神经元之间的交流需要对神经活动进行精确的控制。光热调制是一种远程、非遗传、高时空分辨率控制神经活动的技术。光热活性纳米材料在光照作用下的局部热释放会改变界面神经元的膜性质。近年来，研究表明二维Ti3C2TxMXene是一种具有低入射能量的光热激发神经元的优秀候选材料。然而，使用ti3c2tx3进行神经调节的安全性尚不清楚。本文研究了Ti3C2Tx - based光热调制的生物安全性，包括质膜完整性、线粒体应激和氧化应激的评估。结果表明，在25µg cm−2ti3c2tx薄膜上培养神经元，用不同入射能量（2-10µJ /脉冲）和不同脉冲频率（1脉冲、1 Hz和10 Hz）的激光脉冲（635 nm）照射神经元，既不会损伤细胞膜，也不会诱导细胞应激，也不会产生氧化应激。造成损伤的阈值能量（即每脉冲14µJ）超过了神经激发的入射能量（每脉冲<10µJ）。这种多分析安全性评估为指导光热调制临床翻译的光照条件和方案的建立提供了重要的见解。
Understanding the communication of individual neurons necessitates precise control of neural activity. Photothermal modulation is a remote and non‐genetic technique to control neural activity with high spatiotemporal resolution. The local heat release by photothermally active nanomaterial will change the membrane properties of the interfaced neurons during light illumination. Recently, it is demonstrated that the two‐dimensional Ti3C2TxMXene is an outstanding candidate to photothermally excite neurons with low incident energy. However, the safety of using Ti3C2Txfor neural modulation is unknown. Here, the biosafety of Ti3C2Tx‐based photothermal modulation is thoroughly investigated, including assessments of plasma membrane integrity, mitochondrial stress, and oxidative stress. It is demonstrated that culturing neurons on 25 µg cm−2Ti3C2Txfilms and illuminating them with laser pulses (635 nm) with different incident energies (2–10 µJ per pulse) and different pulse frequencies (1 pulse, 1 Hz, and 10 Hz) neither damage the cell membrane, induce cellular stress, nor generate oxidative stress. The threshold energy to cause damage (i.e., 14 µJ per pulse) exceeded the incident energy for neural excitation (<10 µJ per pulse). This multi‐assay safety evaluation provides crucial insights for guiding the establishment of light conditions and protocols in the clinical translation of photothermal modulation.