Event-averaged measurements of single-molecule kinetics
Event-averaged measurements of single-molecule kinetics
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DOI：
10.1016/s0009-2614(00)00809-5
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发表时间：
2000-09-01
影响因子：
2.8
通讯作者：
Cao, JS
中科院分区：
文献类型：
--
作者：
Cao, JS
调制的 N 构象通道反应系统用于模拟最近的单分子酶实验 [Science 282 (1998) 1877]。模型系统的动力学分析清楚地证明了与全反应的群体动态相关的整体平均浩克测量和与一系列半反应相关的事件平均单分子测量之间的本质区别。双构象通道系统的计算示例支持了所报告实验的主要发现。特别是，对单事件分布函数中的焦点时间和双事件分布函数中的回波信号的观察揭示了构象景观的本质。 (C) 2000 Elsevier Science B.V. 保留所有权利。
A modulated N-conformational-channel reactive system is used to model the recent single-molecule enzymatic experiment [Science 282 (1998) 1877]. Kinetic analysis of the model system clearly demonstrates the essential difference between ensemble-averaged hulk measurements associated with the population dynamics of full-reactions and event-averaged single-molecule measurements associated with a sequence of half-reactions. Example calculations of a two-conformational-channel system support the principal findings of the reported experiment. In particular, the observation of the focal time in the single-event distribution function and the echo signal in the two-event distribution function reveals the nature of conformational landscapes. (C) 2000 Elsevier Science B.V. All rights reserved.