Upregulation of the C/EBP β LAP isoform could be due to decreased TNFAIP3/TNIP1 expression in the peripheral blood mononuclear cells of patients with systemic lupus erythematosus
Upregulation of the C/EBP β LAP isoform could be due to decreased TNFAIP3/TNIP1 expression in the peripheral blood mononuclear cells of patients with systemic lupus erythematosus
复制标题
C/EBP β LAP 亚型的上调可能是由于系统性红斑狼疮患者外周血单核细胞中 TNFAIP3/TNIP1 表达降低所致
DOI：
10.1080/14397595.2016.1232331
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发表时间：
2017-01-01
影响因子：
2.2
通讯作者：
Zhang, Dongmei
中科院分区：
文献类型：
--
作者：
Qian, Tian;Chen, Fangru;Zhang, Dongmei
目的：检测CCAAT/增强子结合蛋白（C/EBP）、tnf α诱导蛋白3 （TNFAIP3）和TNFAIP3相互作用蛋白1 （TNIP1）在系统性红斑狼疮（SLE）患者外周血单个核细胞（PBMCs）中的表达，以评估它们在SLE发病机制中的关系。方法：采用免疫印迹法检测20例SLE患者和20例对照患者外周血中C/EBP、TNIP1和TNFAIP3的表达。通过Spearman分级确定C/EBP /TNFAIP3/TNIP1表达与SLE疾病活动性的相关性。western blotting检测THP-1细胞、转染编码TNFAIP3 shRNA质粒的THP-1细胞和转染编码TNIP1 shRNA的慢病毒载体的THP-1细胞中C/EBP、TNIP1和TNFAIP3的水平。结果：SLE患者C/EBP LAP异构体表达升高，LIP/TNFAIP3/TNIP1表达降低。LAP表达与SLE疾病活动度呈正相关；TNFAIP3和TNIP1的表达与SLE疾病活动性呈负相关。在蛋白尿和抗dsdna抗体升高的SLE患者中，以及在编码TNFAIP3 shRNA的质粒转染的THP-1细胞和编码TNIP1 shRNA的慢病毒载体感染的THP-1细胞中，LAP表达增加。结论：C/EBP /TNFAIP3/TNIP1与SLE活动性相关。C/EBP LAP表达上调可能与TNFAIP3/TNIP1表达降低有关。
Objectives: We aimed to examine CCAAT/enhancer-binding protein (C/EBP ), TNF-alpha-induced protein 3 (TNFAIP3), and TNFAIP3-interacting protein 1 (TNIP1) expression in peripheral blood mononuclear cells (PBMCs) of systemic lupus erythematosus (SLE) patients to assess their relationship in SLE pathogenesis.Methods: C/EBP , TNIP1, and TNFAIP3 expression was assessed in PBMCs from 20 SLE patients and 20 controls by western blotting. The correlation between C/EBP /TNFAIP3/TNIP1 expression and SLE disease activity was determined by Spearman's rank. C/EBP , TNIP1, and TNFAIP3 levels in THP-1 cells, THP-1 cells transfected with plasmids encoding TNFAIP3 shRNA, and THP-1 cells infected with lentiviral vectors encoding TNIP1 shRNA were assessed by western blotting.Results: C/EBP LAP isoform expression was increased and LIP/TNFAIP3/TNIP1 expression was decreased in SLE patients. LAP expression was positively correlated with SLE disease activity; TNFAIP3 and TNIP1 expression was negatively correlated with SLE disease activity. LAP expression was increased in SLE patients with proteinuria and elevated anti-dsDNA antibody, as well as in THP-1 cells transfected with plasmids encoding TNFAIP3 shRNA and THP-1 cells infected with lentiviral vectors encoding TNIP1 shRNA.Conclusions: C/EBP /TNFAIP3/TNIP1 is associated with SLE activity. The upregulated expression of C/EBP LAP could be caused by reduced TNFAIP3/TNIP1 expression.