The occurrence of N-acetyl- and N-glycoloylneuraminic acid in Trypanosoma cruzi.
The occurrence of N-acetyl- and N-glycoloylneuraminic acid in Trypanosoma cruzi.
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DOI：
10.1515/bchm2.1983.364.2.1053
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发表时间：
1983-07
期刊：
影响因子：
--
通讯作者：
R. Schauer;G. Reuter;H. Mühlpfordt;A. F. Andrade;M. Pereira
中科院分区：
文献类型：
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作者：
R. Schauer;G. Reuter;H. Mühlpfordt;A. F. Andrade;M. Pereira
对克氏锥虫的不同菌株进行分析，每10（10）个细胞具有65- 105微克唾液酸。通过薄层色谱法，部分通过气相液相色谱法和气相-液相色谱-质谱法，发现所有菌株均含有不同比例的N-乙酰基-和N-乙醇酰神经氨酸。将寄生虫与[3 H]乙酸盐或N-乙酰基-[3 H]甘露糖胺孵育后，在唾液酸中未发现放射性，因此提示寄生虫无法从其前体合成唾液酸。
Different strain of Trypanosoma cruzi were analysed to have 65--105 micrograms of sialic acids per 10(10) cells. By thin-layer chromatography, and in part by gas liquid chromatography and gas-liquid chromatography-mass spectrometry, all strains were found to contain N-acetyl- and N-glycoloylneuraminic acid in various ratios. After incubation of the parasites with either [3H]acetate or N-acetyl-[3H]mannosamine, no radioactivity was found in the sialic acids, thus leading to the suggestion that the parasites are unable to synthesize sialic acids from their precursors.