Attachment of protein affinity-labeling reagents of variable length and amino acid specificity to E. coli tRNAfMet.
Attachment of protein affinity-labeling reagents of variable length and amino acid specificity to E. coli tRNAfMet.
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将可变长度和氨基酸特异性的蛋白质亲和标记试剂附着到大肠杆菌 tRNAfMet。
DOI：
10.1093/nar/9.5.1203
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发表时间：
1981
影响因子：
14.9
通讯作者：
Reines,SA
中科院分区：
文献类型：
--
作者：
Schulman,LH;Pelka,H;Reines,SA
在亚硫酸氢盐存在下用双官能胺进行转氨基作用已被用于将可变长度的侧链连接到单链胞苷残基inE. colitRNAfMet的N4-位置。这样的侧链，终止于反应性伯氨基，已被偶联到各种N-羟基琥珀酰亚胺酯。所得到的修饰的tRNA携带能够与各种氨基酸共价反应的蛋白质亲和标记基团。
Transamination with bifunctional amines in the presence of bisulfite has been used to attach side chains of variable length to the N4-position of single stranded cytidine residues inE.colitRNAfMet. Such side chains, terminating in reactive primary amino groups, have been coupled to a variety of N-hydroxysuccinimide esters. The resulting modified tRNAs carry protein affinity labeling groups capable of covalent reaction with a variety of amino acids.