Correlation between viability of pollination and length of basal dehiscence of the theca in rice under a hot-and-humid condition
Correlation between viability of pollination and length of basal dehiscence of the theca in rice under a hot-and-humid condition
复制标题
DOI：
10.1626/pps.8.109
复制
发表时间：
2005-06-01
影响因子：
2.5
通讯作者：
Horie, T
中科院分区：
文献类型：
--
作者：
Matsui, T;Kobayasi, K;Horie, T
预期的全球变暖可能会增加水稻（Oryza sativa L.）小花不育性。为了筛选花期耐高温基因型，鉴定与耐高温胁迫相关的形态性状是重要的。本研究以18个水稻品种为材料，在高温高湿（37/25 ℃，昼夜，相对湿度> 90%）条件下，研究了水稻开花期囊基部开裂长度与授粉活力的关系。将对照植物在环境条件下放置在用粗棉布覆盖的半圆柱形房屋中（30%遮蔽;温度范围：24-35 ° C）。开花后，在光学显微镜下观察了柱头上的花粉粒数和囊基部开裂的长度。在常温条件下，小花长度与柱头上80粒以上花粉的百分率呈极显著正相关（r = 0.72，P < 0.001）;在高温条件下，小花长度与柱头上20粒以上花粉的百分率呈极显著正相关（r = 0.93，P < 0.001）。换句话说，在这两种条件下，长度与授粉生活力或可靠性相关。此外，在这两种条件下，非粳型品种的基部开裂比许多粳型品种短。因此，非粳型品种的授粉活力低与其囊壁基部开裂小有关，基部开裂长度可作为耐高温性的选择标记。
Anticipated global warming may increase the floret sterility of rice (Oryza sativa L.). For selection of genotypes tolerant to high temperatures during the flowering period, it is important to identify morphological traits associated with tolerance to temperature stress. This Study investigated the relationship between the length of dehiscence at the basal part of thecae and the viability of pollination in 18 cultivars of rice subjected to a hot-and-humid condition (37/25 degrees C, day/night, > 90% relative humidity) for three days at flowering. Control plants were left under the ambient conditions in a semi-cylindrical house covered with cheesecloth (30% shading; temperature range: 24-35 degrees C). The length of basal dehiscence of thecae and the number of pollen grains on the stigmata were examined with a light microscope after flowering. The length strongly correlated with the percentage of florets having more than 80 pollen grains on the stigmata under the ambient condition (r = 0.72, P < 0.001), and with the percentage of florets having more than 20 pollen grains on the stigmata tinder the hot condition (r = 0.93, P < 0.001). In other words, the length correlated with pollination viability or reliability under both conditions. In addition, basal dehiscence was shorter in the non-japonica-type cultivars than in many of the japonica-type cultivars under both conditions. We concluded that the low pollination viability in the non-japonica, type cultivars is associated with their small basal dehiscence on the thecae, and the length of basal dehiscence can be used as a selection marker of high temperature tolerance.