SDF-1 Increases Recruitment of Osteoclast Precursors by Upregulation of Matrix Metalloproteinase-9 Activity
SDF-1 Increases Recruitment of Osteoclast Precursors by Upregulation of Matrix Metalloproteinase-9 Activity
复制标题
DOI：
10.1080/03008200390152133
复制
发表时间：
2003-01
影响因子：
2.9
通讯作者：
Xuefeng Yu;P. Collin‐Osdoby;P. Osdoby
中科院分区：
文献类型：
--
作者：
Xuefeng Yu;P. Collin‐Osdoby;P. Osdoby
虽然趋化因子在许多循环造血细胞类型的运输和归巢中发挥重要作用，但它们对破骨细胞（OC）募集或骨重塑的潜在影响尚不清楚。因此，通过RNase保护实验分析RANKL在小鼠单核细胞系（RAW 264.7）诱导OC形成过程中趋化因子受体的表达。在RAW细胞（预oc）中检测到相对较高的CXCR4表达，而在RAW- oc发育过程中CXCR4水平下调。CXCR4的独特配体SDF-1刺激RAW细胞产生基质金属蛋白酶(MMP)-9活性，基质蛋白酶是oc前期迁移到发育中的骨髓腔所必需的基质降解酶。RAW细胞中诱导的MMP-9活性与响应于SDF-1的胶原凝胶中mmp依赖性转运增加有关。我们得出结论，SDF-1刺激前OC中MMP-9活性可能是它们向骨募集和在骨髓内迁移到OC分化和骨吸收部位的关键方面。
Although chemokines play essential roles in the trafficking and homing of many circulating hematopoietic cell types, their potential influences on osteoclast (OC) recruitment or bone remodeling are not well known. Therefore, chemokine receptor expression was analyzed by RNase protection assay during OC formation induced by RANKL in a murine mononuclear cell line (RAW 264.7). Relatively high CXCR4 expression was detected in RAW cells (pre-OCs), whereas CXCR4 levels were downregulated during RAW-OC development. SDF-1, the unique ligand for CXCR4, stimulated RAW cell production of matrix metalloproteinase (MMP)-9 activity, a matrix-degrading enzyme essential for pre-OC migration into the developing bone marrow cavity. Induced MMP-9 activity in RAW cells was associated with their increased MMP-dependent transmigration through a collagen gel in response to SDF-1. We conclude that SDF-1 stimulation of MMP-9 activity in pre-OCs may be a key aspect of their recruitment to bone and migration within the marrow to sites for OC differentiation and bone resorption.