Tomato pollen respiration in relation to in vitro germination and pollen tube growth under favourable and stress-inducing temperatures
Tomato pollen respiration in relation to in vitro germination and pollen tube growth under favourable and stress-inducing temperatures
复制标题
DOI：
10.1007/s00497-009-0132-1
复制
发表时间：
2010-09-01
影响因子：
--
通讯作者：
Passam, Harold Christopher
中科院分区：
文献类型：
--
作者：
Karapanos, I. C.;Akoumianakis, K. A.;Passam, Harold Christopher
在15-35A的温度范围内，在液体介质中培养7h，记录了番茄花粉萌发、花粉管生长和呼吸活性。虽然30A的初始呼吸速率最高，但由于高温对萌发和花粉管生长的影响，30A和35A的呼吸作用在培养1小时后都有所下降。7小时内花粉总萌发率在15A℃时达到最大值，而花粉管长度在25A℃时达到最大值。虽然整个孵化期每小时测量的CO2产生量与花粉萌发率或花粉管长度之间没有统计意义上的相关性，但从孵化开始后2小时开始，它与萌发率x花粉管长度的值密切相关，表明在给定的时间内，番茄花粉的呼吸活性是萌发率和花粉管生长的函数。因此，我们认为，呼吸速率可能比简单的萌发试验更好地评价花粉萌发能力，因为它不仅反映了花粉萌发的潜力，而且还反映了花粉管的生长活力。此外，如果体外试验的目的是评估花粉萌发与温度的相互作用，它们应该采用较长的孵化期(例如7小时)，以允许观察到对温度的敏感性差异。
Tomato pollen germination, pollen tube growth and respiratory activity were recorded during incubation in a liquid medium for 7 h over a temperature range of 15-35A degrees C. Although the initial rate of respiration was highest at 30A degrees C, both at 30A degrees C and 35A degrees C respiration decreased after the first hour of incubation due to high temperature impairment of germination and pollen tube growth. The total per cent germination of pollen over the 7-h period was maximal at 15A degrees C whereas pollen tube length was maximal at 25A degrees C. Although the production of CO2 measured at hourly intervals throughout the incubation period did not correlate to a statistically significant level with either the per cent pollen germination or the length of the pollen tubes alone, nevertheless from 2 h after the start of incubation, it closely correlated with the values for germination x pollen tube length, indicating that the respiratory activity of tomato pollen at a given time is a function of both the per cent germination and the pollen tube growth. We suggest therefore that the rate of respiration might be preferable to a simple germination test for the assessment of pollen germination ability since it expresses not only the pollen germination potential but also the growth vigour of the pollen tubes. In addition, where in vitro tests are designed to assess pollen germination-temperature interactions, they should employ a long incubation period (e.g. 7 h) to permit differences in sensitivity to temperature to be observed.