HLA-D types and serum IgG responses to Capnocytophaga in diabetes and periodontitis
HLA-D types and serum IgG responses to Capnocytophaga in diabetes and periodontitis
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DOI：
10.1177/00220345970760120401
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发表时间：
1997-12-01
影响因子：
7.6
通讯作者：
Patil, KD
中科院分区：
文献类型：
--
作者：
Dyer, JK;Peck, MA;Patil, KD
研究了伴有和不伴有1型糖尿病的牙周健康和牙周炎受试者（n = 60）血清中IgG对痰嗜糖细胞噬菌、紫嗜糖细胞噬菌和牙龈嗜糖细胞噬菌细胞包膜蛋白（cep）的反应。采用生物素-山羊抗人IgG和碱性磷酸酶-链亲和素系统免疫印迹法检测血清IgG对cep的反应。通过透射密度测定、数字化和计算机操作分析反应性。糖尿病患者对14种CEP （81 ~ 10 kDa）、5种CEP （90 ~ 17 kDa）和27 kDa CEP的应答显著低于非糖尿病组（p < 0.01）。与牙周健康组相比，牙周炎患者对25- kda、11-kDa、125- kda、17-kDa CEP、42-kDa CEP的反应明显减少（p < 0.01）。HLA-DR4、HLA-DR53和HLA-DQw3与牙周炎相关，而只有HLA-DR4与糖尿病相关（p < 0.02）。非糖尿病患者的HLA-DR2和IgG反应模式以及糖尿病和牙周炎患者的HLA-DR4和IgG反应模式之间存在显著相关性（p < 0.01）。这些结果表明，1型糖尿病患者和牙周炎患者对碳吞噬的IgG抗体谱都较低，这可能是牙周炎感染易感性增加的原因。1型糖尿病患者的牙周炎可能与HLA-D型和免疫功能改变有关，而不是与糖尿病本身有关。
Serum IgG responses to the cell envelope proteins (CEPs) from Capnocytophaga sputigena, Capnocytophaga ochracea, and Capnocytophaga gingivalis were examined in periodontally healthy and periodontitis subjects, both with and without type 1 diabetes (n = 60). Serum IgG responses to CEPs were determined by immunoblotting with biotin-goat anti-human IgG and an alkaline phosphatase-streptavidin system. Reactivity was analyzed by transmission densitometry, digitization, and computer manipulation. The patients with diabetes showed significantly (p < 0.01) fewer responses to 14 CEPs (from 81 to 10 kDa) from C. sputigena, 5 CEPs (from 90 to 17 kDa) from C. gingivalis, and the 27-kDa CEP from C. ochracea than in the non-diabetic group. The periodontitis patients showed significantly (p < 0.01) fewer responses to the 25- and 11-kDa CEPs from C. sputigena, the 125- and 17-kDa CEPs from C. gingivalis, and the 42-kDa CEP from C. ochracea than in the periodontally healthy group. HLA-DR4, HLA-DR53, and HLA-DQw3 were associated with periodontitis, while only HLA-DR4 was associated with diabetes (p < 0.02). Significant (p < 0.01) correlations were found between HLA-DR2 and IgG reactivity patterns associated with non-diabetics, and between HLA-DR4 and IgG reactivity patterns associated with diabetic and periodontitis subjects. These results indicate that both type 1 diabetics and periodontitis subjects have a depressed IgG antibody profile to Capnocytophaga, which may account for an increased susceptibility to periodontitis infection. Periodontitis in type 1 diabetes may be related more to the HLA-D type and altered immune function than to the diabetes itself.