A New Acyl-homoserine Lactone Molecule Generated by Nitrobacter winogradskyi.
A New Acyl-homoserine Lactone Molecule Generated by Nitrobacter winogradskyi.
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维诺格拉茨基硝化杆菌产生的新酰基高丝氨酸内酯分子
DOI：
10.1038/srep22903
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发表时间：
2016-03-11
影响因子：
4.6
通讯作者：
Zhuang G
中科院分区：
文献类型：
--
作者：
Shen Q;Gao J;Liu J;Liu S;Liu Z;Wang Y;Guo B;Zhuang X;Zhuang G
揭示硝化作用的调控机制对于理解农业系统和污水处理中氮素转化具有重要意义。本研究通过在大肠杆菌中异源表达，证实了温氏硝化杆菌nwiI基因是一种高丝氨酸内酯合成酶，可合成多个含7 ~ 11个碳酰基的酰基-高丝氨酸内酯信号。在N. winogradskyi和重组E.杆菌此外，这种新的信号还引发了硝化速率和硝化过程中所涉及的基因的转录水平的变化。这些结果表明，群体感应可能在调节氮代谢的潜在作用。
It is crucial to reveal the regulatory mechanism of nitrification to understand nitrogen conversion in agricultural systems and wastewater treatment. In this study, the nwiI gene of Nitrobacter winogradskyi was confirmed to be a homoserine lactone synthase by heterologous expression in Escherichia coli that synthesized several acyl-homoserine lactone signals with 7 to 11 carbon acyl groups. A novel signal, 7, 8-trans-N-(decanoyl) homoserine lactone (C10:1-HSL), was identified in both N. winogradskyi and the recombined E. coli. Furthermore, this novel signal also triggered variances in the nitrification rate and the level of transcripts for the genes involved in the nitrification process. These results indicate that quorum sensing may have a potential role in regulating nitrogen metabolism.