Phosphorylation and activation of tyrosine hydroxylase mediate the acetylcholine-induced increase in catecholamine biosynthesis in adrenal chromaffin cells.
Phosphorylation and activation of tyrosine hydroxylase mediate the acetylcholine-induced increase in catecholamine biosynthesis in adrenal chromaffin cells.
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DOI：
10.1016/s0021-9258(18)33559-2
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发表时间：
1982-11
期刊：
影响因子：
--
通讯作者：
J. Haycock;J. Meligeni;W. Bennett;J. Waymire
中科院分区：
文献类型：
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作者：
J. Haycock;J. Meligeni;W. Bennett;J. Waymire
乙酰胆碱，天然促分泌素，增加分离纯化的牛肾上腺嗜铬细胞中的儿茶酚胺生物合成。这种作用是钙依赖性的，模仿其他促分泌素，如氨甲酰胆碱，藜芦碱，高KCl，和A23187，和拮抗去极化依赖性钙内流的阻断剂（MnCL，MgCl 2，DBOO）。乙酰胆碱不影响原位3，4-二羟基苯丙氨酸脱羧的速率，并且无论是升高的酪氨酸（100 μ M）、外源性蝶呤（1 mM）还是外源性儿茶酚胺（高达1 mM）都不减弱乙酰胆碱产生的儿茶酚胺生物合成的增加。酪氨酸羟化酶的活性，在体外超声处理后的细胞，增加了嗜铬细胞与乙酰胆碱预先治疗。并且，乙酰胆碱增加32 P掺入酪氨酸羟化酶在完整的嗜铬细胞内预孵育与32 Pi。该酶的活化需要细胞外钙，并且该酶的磷酸化被MnCl 2或乙二醇双（氨乙基醚）-N，N，N ′-四乙酸抑制。
Acetylcholine, the natural secretagogue, increased catecholamine biosynthesis in isolated purified bovine adrenal chromaffin cells. This effect was calcium dependent, mimicked by other secretagogues such as carbamyl choline, veratridine, elevated KCI, and A23187, and antagonized by blockers of depolarization-dependent calcium influx (MnCL, MgC12, DBOO). Acetylcholine did not influence the rate of 3, 4-dihydroxyphenylalanine decarboxylation in situ, and neither elevated tyrosine (100 PM), exogenous pterin (1 mM), nor exogenous catecholamines (up to 1 m) attenuated the increase in catecholamine biosynthesis produced by acetylcholine. Tyrosine hydroxylase activity, measured in vitro after sonication of the cells, was increased by prior treatment of the chromaffin cells with acetylcholine. And, acetylcholine increased 32P incorporation into tyrosine hydroxylase within intact chromaffin cells preincubated with 32Pi. The activation of the enzyme required extracellular calcium, and the phosphorylation of the enzyme was inhibited by either MnClz or ethylene glycol his@-aminoethyl ether)-NjVJV’&” tetraacetic acid.