COLLISION CROSS-SECTIONS FOR PROTEIN IONS
COLLISION CROSS-SECTIONS FOR PROTEIN IONS
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DOI：
10.1016/1044-0305(93)85025-s
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发表时间：
1993-08-01
影响因子：
3.2
通讯作者：
DOUGLAS, DJ
中科院分区：
文献类型：
--
作者：
COVEY, T;DOUGLAS, DJ
本文介绍了一种基于离子通过碰撞气体时的能量损失来测定气相蛋白质离子截面的方法。一个简单的模型将能量损失与碰撞次数以及横截面联系起来。从蒙特卡洛模型，支持这种方法的有效性的结果进行了说明。实验截面胃动素，泛素，细胞色素c肌红蛋白，牛血清白蛋白。横截面的范围从胃动素的约800埃2到牛血清白蛋白的约14，000埃2，并且通常随着离子上的电荷数而增加。细胞色素c离子从水溶液中显示出较小的横截面比从较高的有机含量的溶液中形成的离子，这表明气相离子可能会保留一些记忆，他们的溶液构象。
A method for the determination of cross sections for gas-phase protein ions, based on the energy loss of ions as they pass through a collision gas, is described. A simple model relates the energy loss to the number of collisions and hence the cross section. Results from a Monte Carlo model that support the validity of this approach are described. Experimental cross sections are reported for motilin, ubiquitin, cytochrome c myoglobin, and bovine serum albumin. Cross sections range from approximately 800 angstrom2 for motilin to approximately 14,000 angstrom2 for bovine serum albumin and generally increase with the number of charges on the ion. Cytochrome c ions from aqueous solution show somewhat smaller cross sections than ions formed from solutions of higher organic content, suggesting that the gas-phase ions may retain some memory of their solution conformation.