Dimethoxycurcumin, a Synthetic Curcumin Analogue, Induces Heme Oxygenase-1 Expression through Nrf2 Activation in RAW264.7 Macrophages.
Dimethoxycurcumin, a Synthetic Curcumin Analogue, Induces Heme Oxygenase-1 Expression through Nrf2 Activation in RAW264.7 Macrophages.
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DOI：
10.3164/jcbn.08-194
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发表时间：
2009-01
影响因子：
2.4
通讯作者：
Pae, Hyun-Ock
中科院分区：
文献类型：
--
作者：
Jeong, Sun-Oh;Oh, Gi-Su;Ha, Hun-Yong;Koo, Bon Soon;Kim, Hak Sung;Kim, Youn-Chul;Kim, Eun-Cheol;Lee, Kang-Min;Chung, Hun-Taeg;Pae, Hyun-Ock
关键词：
姜黄素[1，7-双（4-羟基-3-甲氧基苯基）-1，6-庚二酮-3，5-二酮]通过激活核因子-红细胞2相关因子2（Nrf 2）诱导血红素加氧酶-1（HO-1）表达，而姜黄素体内代谢产物之一四氢姜黄素[1，7-双（4-羟基-3-甲氧基苯基）-3，5-庚二酮]对HO-1表达和Nrf 2激活没有影响。本研究的目的是研究二甲氧基姜黄素[1，7-双（4，3-二甲氧基苯基）-1，6-庚烯-3，5-二酮]（一种比姜黄素具有更高代谢稳定性的合成姜黄素类似物）是否能诱导RAW 264. 7巨噬细胞中HO-1表达达到与姜黄素相同的程度。二甲氧基姜黄素和姜黄素，但不是四氢姜黄素，诱导HO-1表达和Nrf 2核转位，这表明化合物的二芳基庚烷链的不饱和性质是至关重要的HO-1表达和Nrf 2激活。通过小干扰RNA阻断Nrf 2的合成，通过二甲氧基姜黄素阻断HO-1的表达，表明二甲氧基姜黄素可能通过Nrf 2激活诱导HO-1的表达。相比之下，二甲氧基姜黄素和姜黄素对HO-1表达具有大致相同的作用，表明二甲氧基姜黄素保留了其母体化合物姜黄素在RAW 264.7巨噬细胞中的HO-1诱导活性。
Curcumin [1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione] induces heme oxygenase-1 (HO-1) expression via activation of the nuclear factor-erythroid-2-related factor 2 (Nrf2), whereas tetrahydrocurcumin [1,7-bis(4-hydroxy-3-methoxyphenyl)-3,5-heptanedione], one of curcumin in vivo metabolites, has no effect on HO-1 expression and Nrf2 activation. The aim of this study was to investigate whether dimethoxycurcumin [1,7-bis(4,3-dimethoxyphenyl)-1,6-heptadiene-3,5-dione], a synthetic curcumin analogue with higher metabolic stability over curcumin, could induce HO-1 expression to the same extent as curcumin in RAW264.7 macrophages. Dimethoxycurcumin and curcumin, but not tetrahydrocurcumin, induced HO-1 expression and Nrf2 nuclear translocation, suggesting that the unsaturated nature of the diarylheptanoid chain of the compounds are crucial for HO-1 expression and Nrf2 activation. Blockage of Nrf2 synthesis by small interfering RNA abolished HO-1 expression by dimethoxycurcumin, indicating that dimethoxycurcumin may induce HO-1 expression via Nrf2 activation. In comparison, dimethoxycurcumin and curcumin had about the same effect on HO-1 expression, suggesting that dimethoxycurcumin retains the HO-1-inducing activity of its parent compound curcumin in RAW264.7 macrophages.
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影响因子：
11.5
作者：
Tamvakopoulos, Constantin;Dimas, Konstantinos;Pantazis, Panayotis
通讯作者：
Pantazis, Panayotis
影响因子：
4.1
作者：
Balogun, E;Hoque, M;Motterlini, R
通讯作者：
Motterlini, R
DOI：
10.1016/j.bbrc.2006.12.133
发表时间：
2007-02-23
影响因子：
3.1
作者：
Pae, Hyun-Ock;Jeong, Sun-Oh;Chung, Hun-Taeg
通讯作者：
Chung, Hun-Taeg
影响因子：
12.8
作者：
Pae, Hyun-Ock;Jeong, Gil-Saeng;Chung, Hun-Taeg
通讯作者：
Chung, Hun-Taeg
影响因子：
12.8
作者：
Jeong, Gil-Saeng;Oh, Gi-Su;Chung, Hun-Taeg
通讯作者：
Chung, Hun-Taeg