Yersiniophage φR1-37 is a tailed bacteriophage having a 270 kb DNA genome with thymidine replaced by deoxyuridine
Yersiniophage φR1-37 is a tailed bacteriophage having a 270 kb DNA genome with thymidine replaced by deoxyuridine
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DOI：
10.1099/mic.0.28265-0
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发表时间：
2005-12-01
期刊：
影响因子：
2.8
通讯作者：
Skurnik, M
中科院分区：
文献类型：
--
作者：
Kiljunen, S;Hakala, K;Skurnik, M
噬菌体phi R1-37是根据其感染菌株YeO（3）-R1的能力分离的，YeO（3）-R1是小肠结肠炎耶尔森氏菌血清型O：3的毒力质粒治愈的O抗原阴性衍生物。在这项研究中，噬菌体受体被发现是在Y的外核心六糖的结构。小肠结肠炎O：3 LPS。噬菌体受体存在于许多其他Y.小肠结肠炎血清型，但也在一些中间耶尔森氏菌菌株。令人惊讶的是，受体结构存在于假结核耶尔森氏菌O：9的O抗原中。电子显微镜显示，phi R1-37颗粒具有88 nm的二十面体头部、10 nm的短颈部、236 nm的长收缩尾部和至少86 nm的尾部纤维。这意味着噬菌体属于ICTV（国际病毒分类委员会）分类中的尾病毒目和肌尾病毒科。发现phi R1-37具有裂解生命周期，其中蚀和潜伏期分别为40和50分钟，并且爆发大小类似于80 p.f.u.。每一个被感染的细胞限制性内切酶分析和脉冲场凝胶电泳结果表明，phi R1-37基因组为双链DNA，大小约为270 kb。对酶水解的DNA进行HPLC-MS/MS分析，这证明phi R1-37基因组由其中胸苷（T）> 99%被脱氧尿苷（dU）取代的DNA组成。已知具有相似DNA的唯一生物体是枯草芽孢杆菌特异性噬菌体PBS 1和PBS 2。四种主要结构蛋白的N-末端氨基酸序列与数据库中的（病毒）蛋白序列没有任何相似性，表明phi R1-37的近亲尚未被鉴定。从部分测序的phi R1-37基因组中鉴定出两种结构蛋白p24和p46的基因。
Bacteriophage phi R1-37 was isolated based on its ability to infect strain YeO(3)-R1, a virulence-plasmid-cured O antigen-negative derivative of Yersinia enterocolitica serotype O : 3. In this study, the phage receptor was found to be a structure in the outer core hexasaccharide of Y. enterocolitica O : 3 LPS. The phage receptor was present in the outer core of strains of many other Y. enterocolitica serotypes, but also in some Yersinia intermedia strains. Surprisingly, the receptor structure resided in the O antigen of Yersinia pseudotuberculosis O : 9. Electron microscopy demonstrated that phi R1-37 particles have an icosahedral head of 88 nm, a short neck of 10 nm, a long contractile tail of 236 nm, and tail fibres of at least 86 nm. This implies that the phage belongs to the order Caudovirales and the family Myoviridae in the ICTV (International Committee for Taxonomy of Viruses) classification. phi R1-37 was found to have a lytic life cycle, with eclipse and latent periods of 40 and 50 min, respectively, and a burst size of similar to 80 p.f.u. per infected cell. Restriction digestions and PFGE showed that the phi R1-37 genome was dsDNA and similar to 270 kb in size. Enzymically hydrolysed DNA was subjected to HPLC-MS/MS analysis, which demonstrated that the phi R1-37 genome is composed of DNA in which thymidine (T) is > 99 % replaced by deoxyuridine (dU). The only organisms known to have similar DNA are the Bacillus subtilis-specific bacteriophages PBS1 and PBS2. N-terminal amino acid sequences of four major structural proteins did not show any similarity to (viral) protein sequences in databases, indicating that close relatives of phi R1-37 have not yet been characterized. Genes for two of the structural proteins, p24 and p46, were identified from the partially sequenced phi R1-37 genome.