Fabrication and Characterization of Natural Origin Chitosan-Gelatin-Alginate Composite Scaffold by Foaming Method Without Using Surfactant
Fabrication and Characterization of Natural Origin Chitosan-Gelatin-Alginate Composite Scaffold by Foaming Method Without Using Surfactant
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DOI：
10.1002/app.37755
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发表时间：
2013-02-15
影响因子：
3
通讯作者：
Mishra, Narayan Chandra
中科院分区：
文献类型：
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作者：
Sharma, Chhavi;Dinda, Amit Kumar;Mishra, Narayan Chandra
采用发泡法，在不添加任何泡沫稳定表面活性剂的情况下，制备了天然来源的壳聚糖、明胶和海藻酸钠三聚体支架。以前，在支架制造的发泡方法中，使用有毒的表面活性剂来稳定泡沫，但在这项工作中，严格避免使用表面活性剂，这可以为细胞反应和活力提供更好的环境。在发泡法中，简单地通过搅拌聚合物（海藻酸盐-明胶）溶液来产生稳定的泡沫，并将泡沫与CaCl 2、戊二醛和壳聚糖交联以产生三聚体海藻酸盐-明胶-壳聚糖复合支架。复合支架的显微镜图像显示存在互连孔，大部分分布在支架的整个表面上。该支架具有90%的孔隙率，平均孔径为57 μ m。溶胀和降解研究表明，该支架具有良好的亲水性和生物降解性。通过在支架上接种L929小鼠成纤维细胞进行的体外细胞培养研究显示，如MTT测定、DNA定量和细胞-支架构建物的相差显微镜所示，细胞活力、增殖率和粘附性优异。通过最简单的方法制造的天然来源的复合支架，发泡法不添加任何表面活性剂，成本低廉，生物相容性好，在组织工程领域有潜在的应用前景。(C)2012 Wiley Periodicals，Inc. J.应用聚合物Sci. 127：3228-3241，2013
A natural origin tripolymer scaffold from chitosan, gelatin, and alginate was fabricated by applying foaming method without adding any foam stabilizing surfactant. Previously, in foaming method of scaffold fabrication, toxic surfactants were used to stabilize the foam, but in this work, the use of surfactant has been avoided strictly, which can provide better environment for cellular response and viability. In foaming method, stable foam is produced simply by agitating the polymer (alginate-gelatin) solution, and the foam is crosslinked with CaCl2, glutaraldehyde, and chitosan to produce tripolymer alginate-gelatin-chitosan composite scaffold. Microscopic images of the composite scaffold revealed the presence of interconnected pores, mostly spread over the entire surface of the scaffold. The scaffold has a porosity of 90% with a mean pore size of 57 mu m. Swelling and degradation studies of the scaffold showed that the scaffold possesses excellent properties of hydrophilicity and biodegradability. In vitro cell culture studies by seeding L929 mouse fibroblast cells on scaffold revealed excellent cell viability, proliferation rate and adhesion as indicated by MTT assay, DNA quantification, and phase contrast microscopy of cell-scaffold construct. The natural origin composite scaffold fabricated by the simplest method i.e., foaming method, but without adding any surfactant, is cheap, biocompatible, and it might find potential applications in the field of tissue engineering. (C) 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 127: 3228-3241, 2013