Gene expression signatures for identifying diffuse-type gastric cancer associated with epithelial-mesenchymal transition
Gene expression signatures for identifying diffuse-type gastric cancer associated with epithelial-mesenchymal transition
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DOI：
10.3892/ijo.2014.2387
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发表时间：
2014-06-01
影响因子：
5.2
通讯作者：
Sasaki, Hiroki
中科院分区：
文献类型：
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作者：
Tanabe, Shihori;Aoyagi, Kazuhiko;Sasaki, Hiroki
上皮-间充质转化(EMT)与肿瘤的恶性程度密切相关。与肠型胃癌相比，Hedgehog-EMT通路在弥漫型胃癌中被优先激活；然而，组织学分型是目前区分这两种主要类型胃癌的唯一方法。我们比较了12个骨髓间充质干细胞培养和5个弥漫型GC组织样本的基因表达谱。在弥漫型胃癌中发现了大量上调或下调的基因，包括CDH1、CDH2、VIM、WNT4和WNT5。其中，cdh2/cdh1的mRNA比值可以区分15例弥漫型和17例肠型胃癌。我们的结果表明，弥漫型GC的间质特征比肠型GC更明显，但弥漫型GC的间质特征弱于间充质干细胞。经过广泛EMT的弥漫型GC预后较差，仅通过两个基因的定量聚合酶链式反应分析即可确诊。
Epithelial-mesenchymal transition (EMT) is associated with tumor malignancy. The hedgehog-EMT pathway is preferentially activated in diffuse-type gastric cancer (GC) compared with intestinal-type GC; however, histological typing is currently the only method for distinguishing these two major types of GC. We compared the gene expression profiles of 12 bone marrow-derived mesenchymal stem cell cultures and 5 diffuse-type GC tissue samples. Numerous upregulated or downregulated genes were identified in diffuse-type GC, including CDH1,CDH2,VIM,WNT4 and WNT5. Among these genes, the mRNA ratio of CDH2 to CDH1 could distinguish the 15 diffuse-type GC samples from the 17 intestinal-type GC samples. Our results suggested that the mesenchymal features were more prominent in diffuse-type GC than in intestinal-type GC, but were weaker in diffuse-type GC than in mesenchymal stem cells. Diffuse-type GC that has undergone extensive EMT, which has a poor prognosis, can be identified by quantitative PCR analysis of only two genes.