T (Brachyury) is a direct target of Wnt3a during paraxial mesoderm specification
T (Brachyury) is a direct target of Wnt3a during paraxial mesoderm specification
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DOI：
10.1101/gad.13.24.3185
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发表时间：
1999-12-15
影响因子：
10.5
通讯作者：
McMahon, AP
中科院分区：
文献类型：
--
作者：
Yamaguchi, TP;Takada, S;McMahon, AP
Wnt 3a编码一个信号，该信号在原肠胚小鼠胚胎的原条中表达，并且是近轴中胚层发育所需的。在它的情况下，细胞采取异位神经命运。缺乏含有T-box的转录因子Brachyury或Tbx 6的胚胎也缺乏近轴中胚层。在这里，我们表明，Brachyury是特别下调Wnt 3a突变体的细胞注定要形成近轴中胚层。T启动子的转基因分析将T（Brachyury）鉴定为Wnt信号传导途径的直接转录靶标。我们的研究结果表明，Wnt 3a，通过Brachyury信号，调节原肠胚形成过程中胚层和神经细胞命运之间的平衡。
Wnt3a encodes a signal that is expressed in the primitive streak of the gastrulating mouse embryo and is required for paraxial mesoderm development. In its absence cells adopt ectopic neural fates. Embryos lacking the T-box-containing transcription factors, Brachyury or Tbx6, also lack paraxial mesoderm. Here we show that Brachyury is specifically down-regulated in Wnt3a mutants in cells fated to form paraxial mesoderm. Transgenic analysis of the T promoter identifies T (Brachyury) as a direct transcriptional target of the Wnt signaling pathway. Our results suggest that Wnt3a, signaling via Brachyury, modulates a balance between mesodermal and neural cell fates during gastrulation.