Histone Marks and Chromatin Remodelers on the Regulation of neurogenin1 Gene in RA Induced Neuronal Differentiation of P19 Cells
Histone Marks and Chromatin Remodelers on the Regulation of neurogenin1 Gene in RA Induced Neuronal Differentiation of P19 Cells
复制标题
组蛋白标记和染色质重塑剂对 RA 诱导 P19 细胞神经元分化中神经原蛋白 1 基因的调节
DOI：
10.1002/jcb.22122
复制
发表时间：
2009-05-15
影响因子：
4
通讯作者：
Shen, Yu-fei
中科院分区：
文献类型：
--
作者：
Wu, Meng;Zhang, Ye;Shen, Yu-fei
Neurogenin1是一种重要的bHLH蛋白，在神经发生中起重要作用。我们首先发现ngn1的表达在RA诱导的神经元分化中急剧增加。在此期间，发现p19细胞中围绕ngn1基因的三个连续的表观遗传变化阶段与抑制该基因的激活相关。抑制组蛋白编码H3K27me3在ngn1基因上的募集是第一抑制阶段的主要变化，随后在RA治疗的第二和第三阶段，H3K9ac、H3K14ac和H3K4me3的活性编码结合。此外，BRM而非BRG1在第三阶段特异性募集到ngn1基因，并积极参与RA诱导的ngn1表达。我们认为组蛋白修饰剂和染色质重塑剂在RA诱导的P19细胞分化中ngn1基因的激活中起着关键作用。j .细胞。生物化学学报，26(2):444 - 444,2009。(C) 2009 Wiley-Liss, Inc。
Neurogenin1 is an important bHLH protein that plays crucial role in neurogenesis. We first show that the expression of ngn1 increases drastically in RA induced neuronal differentiation. During which, a three successive stages of the epigenetic changes surrounding the ngn1 gene are found correlated with a repression to activation of the gene in P 19 cells. Recruiting of a repressive histone code H3K27me3 on the ngn1 gene is the dominant change in first repression stage, which is followed by the binding of the active codes of H3K9ac, H3K14ac, and the H3K4me3 in the second and third stages of RA treatment. Additionally, BRM but not BRG1 is specifically recruited to ngn1 gene at the third stage and is positively involved in the RA induced ngn1 expression. We propose that histone modifiers and chromatin remodelers are pivotal in the activation of the ngn1 gene in RA induced differentiation of P19 cells. J. Cell. Biochem. 107: 264-271, 2009. (C) 2009 Wiley-Liss, Inc.