Detection of Helicobacter pylori in gastric biopsy and resection specimens
Detection of Helicobacter pylori in gastric biopsy and resection specimens
复制标题
DOI：
10.1136/jcp.49.2.107
复制
发表时间：
1996-02-01
影响因子：
3.4
通讯作者：
Isaacson, PG
中科院分区：
文献类型：
--
作者：
AshtonKey, M;Diss, TC;Isaacson, PG
目的：比较染色、银染、免疫组织化学和聚合酶链式反应(PCR)在胃活检和切除标本中检测幽门螺杆菌的敏感性。方法33例胃活检标本(慢性胃炎26例，低级别粘膜相关淋巴组织(MALT)淋巴瘤7例)和胃切除标本(MALT淋巴瘤5例)均经福尔马林固定石蜡包埋。连续切片用苏木精-伊红染色、Giemsa染色、Wartin-Starry银染和热诱导抗原修复后的免疫过氧化物酶技术。结果苏木精伊红染色14例(37%)，Giemsa染色21例(55%)，Warthing-Starry染色23例(61%)，抗体染色25例(66%)。聚合酶链式反应阳性17例(45%)。结论采用免疫组织化学技术结合热诱导抗原修复技术检测胃活检和切除标本中的幽门螺杆菌，具有较高的敏感性和易用性。
Aims-To compare the sensitivity of detecting Helicobacter pylori in gastric biopsy and resection specimens using tinctorial and silver impregnation stains, immunohistochemistry and the polymerase chain reaction (PCR).Methods-Formalin fixed, paraffin wax embedded tissue from 33 gastric biopsy specimens (26 showing chronic gastritis and seven showing low grade mucosa associated lymphoid tissue (MALT) lymphoma) together with blocks of uninvolved mucosa from gastrectomy specimens for MALT lymphoma (five cases) were studied. Consecutive sections were stained using haematoxylin and eosin, Giemsa, the Warthin-Starry silver stain, and a polyclonal antibody directed against H pylori using an immunoperoxidase technique following heat induced antigen retrieval. PCR analysis of DNA extracted from a further section was carried out using primers which amplified a 411 base pair fragment of the urease A gene.Results-H pylori was detected in 14 (37%) sections stained with haematoxylin and eosin, 21 (55%) with Giemsa, 23 (61%) with Warthin-Starry, and 25 (66%) stained with the antibody. Seventeen (45%) cases were positive on PCR. Immunohistochemistry was positive in all cases in which H pylori was detected by other methods.Conclusion-Immunohistochemistry using an immunoperoxidase technique following heat induced antigen retrieval for detecting H pylori in gastric biopsy and resection specimens is highly sensitive and easy to use.