Prevalence of Rickettsia species in ticks including identification of unknown species in two regions in Kazakhstan
Prevalence of Rickettsia species in ticks including identification of unknown species in two regions in Kazakhstan
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DOI：
10.1186/s13071-019-3440-9
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发表时间：
2019-05-03
影响因子：
3.2
通讯作者：
Essbauer, Sandra
中科院分区：
文献类型：
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作者：
Turebekov, Nurkeldi;Abdiyeva, Karlygash;Essbauer, Sandra
60多年前，在哈萨克斯坦首次描述了类似蜱传立克次体病的临床模式。自1995年以来，人类蜱传立克次体病临床病例的发病率似乎在上升，但迄今为止，关于哈萨克斯坦境内发生的病原体、蜱媒物种、流行率和分布的流行病学数据的研究仍然很少。本研究的目的是在克孜勒奥尔达地区和迄今为止被认为是非地方病的阿拉木图地区对蜱进行斑点热群立克次体的分子调查。在哈萨克斯坦的两个地区共收集了2341只蜱，并分类到501个池中：全沟硬蜱（243只）;边缘革蜱（129只）;点状血蜱（104只）;亚洲璃眼蜱（17只）;网纹革蜱（3只）和土耳其扇头蜱（5只）。对样本进行立克次体属检测。使用实时PCR。对于阳性样品多位点序列分型（MLST）performed.ResultsThe计算的最低感染率（MIR）在阿拉木图地区的调查蜱立克次体在克孜勒奥尔达地区的0.4-15.1%和12.6-22.7%之间变化。在哈萨克斯坦的两个选定地区至少发现了四种不同的立克次体。其中两种已经为科学所知：Rickettsia raoultii和R。slovaca，后者为阿拉木图地区首次报道。yenbekshikazakhensis的6个基因片段的MLST和一个新的基因型R。talgarensis检测使用三个基因fragments.ConclusionsKazakh医生应该知道立克次体病蜱叮咬后，在这两个地区的研究。两个，R。raoultii和R.斯洛伐克应被纳入诊断。新发现的立克次氏体在人类疾病中的作用有待进一步研究。yenbekshikazakhensis和R.塔尔加西斯。
BackgroundOver 60 years ago clinical patterns resembling tick-borne rickettsioses have been described for the first time in Kazakhstan. Since 1995 the incidence of clinical cases of tick-borne rickettsioses in humans seems to be rising but studies on epidemiological data regarding the occurring etiological agents, tick vector species, prevalence and distribution throughout Kazakhstan are still scarce to date. The aim of the study was molecular investigation of ticks for spotted-fever group rickettsiae in the endemic Kyzylorda region and the so far considered as non-endemic Almaty region. A total of 2341 ticks was collected in the two regions in Kazakhstan and sorted in 501 pools: Ixodes persulcatus (243); Dermacentor marginatus (129); Haemaphysalis punctata (104); Hyalomma asiaticum (17); Dermacentor reticulatus (3); and Rhipicephalus turanicus (5). Pools were tested for Rickettsia spp. using real-time PCR. For positive samples multilocus sequence typing (MLST) was performed.ResultsThe calculated minimum infection rate (MIR) for rickettsiae in the investigated ticks in Almaty region varied between 0.4-15.1% and 12.6-22.7% in the Kyzylorda region. At least four different Rickettsia species were identified in the two selected regions of Kazakhstan. Two of these are already known to science: Rickettsia raoultii and R. slovaca, the latter being reported for the first time in Almaty region One new form, Candidatus R. yenbekshikazakhensis, was described by MLST of six gene fragments in Almaty region and one new genotype, genotype R. talgarensis was detected using three gene fragments.ConclusionsKazakh physicians should be aware of rickettsioses after tick bites in both regions studied. Both, R. raoultii and R. slovaca should be included in the diagnostics. The role for human diseases has further to be investigated for the newly described rickettsiae, Candidatus R. yenbekshikazakhensis and Genotype R. talgarensis.