Juvenile hormone diol kinase -: II.: Sequencing, cloning, and molecular modeling of juvenile hormone-selective diol kinase from Manduca sexta
Juvenile hormone diol kinase -: II.: Sequencing, cloning, and molecular modeling of juvenile hormone-selective diol kinase from Manduca sexta
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DOI：
10.1074/jbc.m201509200
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发表时间：
2002-06-14
影响因子：
4.8
通讯作者：
Schooley, DA
中科院分区：
文献类型：
--
作者：
Maxwell, RA;Welch, WH;Schooley, DA
烟草天蛾保幼激素二醇激酶（JHDK）的基因序列编码与果蝇肌浆钙结合蛋白-2（dSCP 2）具有59%序列同一性的酶。JHDK和dSCP 2类似于G蛋白，具有参与嘌呤核苷酸结合的三个保守序列元件。这两种蛋白质都含有两对EF-手基序。对D. melanogaster同源物M. Sexta JHDK从成年D.黑腹果蝇的JHDK活性。该活性具有与dSCP 2几乎相同的实验pI和分子量。此外，D.与M.十六进制JHDK。底物对接到JHDK的三维模型已经表明，三个保守的核苷酸结合元件围绕推定的底物结合位点，并与p21（Ras）和腺苷酸激酶的保守序列元件对齐。D. melanogaster dSCP 2是M. sexta JHDK，这些蛋白质构成了一个新的激酶家族，使用SCP（蛋白质数据库代码2SCP）的支架结合核苷酸。
The gene sequence of Manduca sexta juvenile hormone diol kinase (JHDK) codes for an enzyme that has 59% sequence identity to Drosophila melanogaster sarcoplasmic calcium-binding protein-2 (dSCP2). JHDK and dSCP2 are similar to G-proteins with three conserved sequence elements involved in purine nucleotide binding. Both proteins contain two pairs of EF-hand motifs. Characterization and partial purification of the D. melanogaster homolog of M. sexta JHDK from adult D. melanogaster gave material with JHDK activity. This activity has an experimental pI and molecular mass that are nearly identical to those of dSCP2. Moreover, D. melanogaster phosphotransferase activity has very similar chromatographic retention in three systems compared with M. sexta JHDK. Substrate docking to three-dimensional models of JHDK has shown that the three conserved nucleotide-binding elements surround the putative substrate-binding site and align with conserved sequence elements of p21(Ras) and adenylate kinase. D. melanogaster dSCP2 is a homolog of M. sexta JHDK, and these proteins constitute a novel kinase family that binds nucleotides using the scaffold of an SCP (Protein Data Bank code 2SCP).