Epidermal tight junctions:: ZO-1 and occludin are expressed in mature, developing, and affected skin and in vitro differentiating keratinocytes
Epidermal tight junctions:: ZO-1 and occludin are expressed in mature, developing, and affected skin and in vitro differentiating keratinocytes
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DOI：
10.1046/j.0022-202x.2001.01493.x
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发表时间：
2001-11-01
影响因子：
6.5
通讯作者：
Peltonen, S
中科院分区：
文献类型：
--
作者：
Pummi, K;Malminen, M;Peltonen, S
这项研究表明，在成人和发育中的人表皮中存在紧密连接抗原。用ZO-1和occludin抗体进行间接免疫荧光标记和免疫电子显微镜观察，将紧密连接成分ZO-1和occludin定位于成人表皮颗粒细胞的狭窄区域。对紧密连接成分与粘连连接或桥粒蛋白的双重免疫标记表明，occludin对紧密连接比ZO-1更具特异性，ZO-1也可能与粘连连接相关。在发育中的皮肤，紧密连接的周皮细胞，并在胎儿分层后定位于颗粒细胞层。对代表表皮异常分化的银屑病、扁平苔藓和寻常型鱼鳞病的免疫标记表明，这些情况与ZO-1和阻滞素重新定位到棘细胞有关。表皮角质形成细胞的培养为细胞接触的形成提供了一个有用的模型，结果显示紧密连接成分ZO-1和occludin在融合区呈现线性外观的过程中相对较晚地显示出显著的共定位程度。这表明黏附连接和桥粒的形成先于紧密连接的形成。我们推测，将人体与外部环境隔离的表皮屏障，部分是由颗粒层的紧密连接形成的。
This study demonstrates the presence of tight junction antigens in adult and developing human epidermis. Indirect immunofluorescence labeling and immunoelectron microscopy with antibodies to ZO-1 and occludin localized tight junction components ZO-1 and occludin to a narrow zone of the granular cells of adult epidermis. Double immunolabeling for tight junction components with adherens junction or desmosome proteins suggested that occludin is more specific for tight junctions than ZO-1, which may also be associated with adherens junctions. In developing skin, tight junctions interconnected the peridermal cells, and after the fetal stratification localized to the granular cell layer. Immunolabeling of psoriasis, lichen planus, and ichthyosis vulgaris, representing aberrant differentiation of the epidermis, showed that these conditions were associated with relocation of ZO-1 and occludin to the spinous cells. Cultures of epidermal keratinocytes, which offer a useful model for the formation of cellular contacts, revealed that tight junction components, ZO-1 and occludin, displayed a marked degree of colocalization relatively late during the process when the fusion zone had assumed a linear appearance. This suggests that the formation of adherens junctions and desmosomes precedes that of tight junctions. We speculate that the epidermal barrier, isolating the human body from the external environment, is in part formed by tight junctions of stratum granulosum.