The structural organization of the chicken calmodulin gene.
The structural organization of the chicken calmodulin gene.
复制标题
鸡钙调蛋白基因的结构组织。
DOI：
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发表时间：
1985
影响因子：
4.8
通讯作者：
A. Means
中科院分区：
文献类型：
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作者：
R. Simmen;T. Tanaka;K. F. Ts'ui;J. Putkey;M. Scott;E. Lai;A. Means
通过分析从 Charon 4A 和粘粒 DNA 文库获得的重叠基因组克隆，确定了整个鸡钙调蛋白 (CaM) 基因的结构组织。这些克隆总共跨越了 39 KB 的鸡基因组 DNA。 CaM 基因长 12 KB，包含 8 个被大小高度可变的内含子中断的外显子。第一个内含子 (A) 仅 19 个碱基对 (bp) 长，分隔 5' 非翻译区。内含子 B 将 ATG 与编码 CaM (Ala) NH2 末端氨基酸的三联体的第一个核苷酸分开，而内含子 C 将编码氨基酸 10-11 的三联体分开。内含子 D、F 和 G 中断 CaM 的 Ca2+ 结合子结构域 II、III 和 IV，而内含子 E 则位于该蛋白高度同源的 NH2 和 COOH 末端半部之间的连接区中。使用鸡脑 Poly(A+) mRNA 和 CaM cDNA 5' 非翻译区的片段进行引物延伸研究，确定 mRNA 的假定转录起始位点（帽位点）位于距起始密码子 ATG 5' 103 bp 处。共有序列 TATTTAA 位于距该帽位点 5' 29 bp 的位置，而 CCAAT 序列则位于距离该帽位点 5' 更远的 -58 bp 位置。 CaM 基因的结构与编码来自海胆、鸡、大鼠和鹌鹑的其他 Ca2+ 结合蛋白的基因惊人地相似。这些数据表明与钙结合和 Ca2+ 结合蛋白的调节域相关的基因组组织的保守性。
The structural organization of the entire chicken calmodulin (CaM) gene was determined by analysis of overlapping genomic clones obtained from Charon 4A and cosmid DNA libraries. These clones together span 39 kilobases of chicken genomic DNA. The CaM gene is 12 kilobases long and contains 8 exons interrupted by introns of highly variable size. The first intron (A) is only 19 base pairs (bp) long and divides the 5' untranslated region. Intron B separates the ATG from the first nucleotide of the triplet which encodes the NH2-terminal amino acid of CaM (Ala) whereas intron C separates the triplets encoding amino acids 10-11. Introns D, F, and G interrupt the Ca2+ binding subdomains II, III, and IV of CaM whereas intron E is localized in the linker region between the highly homologous NH2- and COOH-terminal halves of the protein. Primer extension studies using chicken brain poly(A+) mRNA and a fragment from the 5' untranslated region of a CaM cDNA identified the presumptive transcription initiation site (cap site) of the mRNA to be 103 bp 5' from the initiation condon ATG. A consensus sequence TATTTAA was localized 29 bp 5' from this cap site while a CCAAT sequence was located further 5' at position -58 bp. The structure of the CaM gene is strikingly similar to genes that encode other Ca2+ binding proteins from sea urchin, chicken, rat, and quail. These data suggest a conservation of genome organization related to the calcium binding and regulatory domains of Ca2+ binding proteins.