In Situ Enhancement and Isotopic Labeling of Biogenic Coalbed Methane
In Situ Enhancement and Isotopic Labeling of Biogenic Coalbed Methane
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DOI：
10.1021/acs.est.1c05979
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发表时间：
2022-03-01
影响因子：
11.4
通讯作者：
Fields, Matthew W.
中科院分区：
文献类型：
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作者：
Barnhart, Elliott P.;Ruppert, Leslie F.;Fields, Matthew W.
地下微生物（生物）甲烷生产是全球碳循环的重要组成部分，导致全球许多煤层中的天然气积聚。实验室研究表明，复杂的含碳营养素（例如，酵母或藻类提取物）可以刺激甲烷产生，但这些营养物在煤层中的有效性尚不清楚。在这里，我们使用井下监测方法结合氘水（D2O）和200升注射0.1%的酵母提取物（YE），以刺激和同位素标记新产生的甲烷。总溶解气体压力传感器实现了实时气体测量（注射前641天和注射后478天）。用地下环境采样器收集的井下样品表明，甲烷比注前水平增加了132%，基于D2O的同位素标记，基于压力读数增加了108%，基于注后266天的甲烷测量增加了183%。证明YE提高生物煤层气生产原位使用多种新的测量方法有直接的影响，其他领域规模的生物甲烷调查，包括原位方法来检测和跟踪微生物活动有关的地下甲烷转化的甲烷的柠檬酸碳。
Subsurface microbial (biogenic) methane production is an important part of the global carbon cycle that has resulted in natural gas accumulations in many coal beds worldwide. Laboratory studies suggest that complex carbon-containing nutrients (e.g., yeast or algae extract) can stimulate methane production, yet the effectiveness of these nutrients within coal beds is unknown. Here, we use downhole monitoring methods in combination with deuterated water (D2O) and a 200-liter injection of 0.1% yeast extract (YE) to stimulate and isotopically label newly generated methane. A total dissolved gas pressure sensor enabled real-time gas measurements (641 days preinjection and for 478 days postinjection). Downhole samples, collected with subsurface environmental samplers, indicate that methane increased 132% above preinjection levels based on isotopic labeling from D2O, 108% based on pressure readings, and 183% based on methane measurements 266 days postinjection. Demonstrating that YE enhances biogenic coalbed methane production in situ using multiple novel measurement methods has immediate implications for other field-scale biogenic methane investigations, including in situ methods to detect and track microbial activities related to the methanogenic turnover of recalcitrant carbon in the subsurface.