The role of aquaporins in dendritic cell macropinocytosis.
The role of aquaporins in dendritic cell macropinocytosis.
复制标题
水通道蛋白在树突状细胞大型细胞增多症中的作用。
DOI：
10.1084/jem.191.4.743
复制
发表时间：
2000-02-21
影响因子：
15.3
通讯作者：
Lanzavecchia, A
中科院分区：
文献类型：
--
作者：
de Baey, A;Lanzavecchia, A
未成熟树突状细胞（DC）通过巨胞饮作用组成性地摄取大量液体，并将大溶质浓缩在内吞区室中。这种浓缩机制是其呈递可溶性抗原的高能力的基础，需要DC能够快速交换穿过其膜的水。我们报道了水通道蛋白家族的两个成员，AQP3和AQP7，在未成熟的DC中表达，并且在成熟后下调。对氯汞苯磺酸盐（pCMBS），一种汞的药物，阻止水通道蛋白，治疗的DCs抑制的吸收和浓度的大溶质所采取的液相内吞作用，并导致显着的细胞肿胀。相反，pCMBS不影响通过甘露糖受体的受体介导的内吞作用。这些研究结果表明，水通道蛋白的体积控制机制，使DC集中通过巨胞饮采取的大溶质的基本要素。
Immature dendritic cells (DCs) constitutively take up large volumes of fluid by macropinocytosis and concentrate the macrosolutes in the endocytic compartment. This concentration mechanism that is the basis of their high capacity to present soluble antigens requires that DCs be capable of rapidly exchanging water across their membranes. We report that two members of the aquaporin family, AQP3 and AQP7, are expressed in immature DCs and are downregulated after maturation. Treatment of DCs with p-chloromercuribenzenesulphonate (pCMBS), a mercuric drug that blocks aquaporins, inhibited uptake and concentration of macrosolutes taken up by fluid phase endocytosis and led to dramatic cell swelling. In contrast, pCMBS did not affect receptor-mediated endocytosis via the mannose receptor. These findings indicate that aquaporins represent essential elements of a volume control mechanism that allows DCs to concentrate macrosolutes taken up via macropinocytosis.