Effect of intermittent feeding on renal hemodynamics in conscious rats.
Effect of intermittent feeding on renal hemodynamics in conscious rats.
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间歇喂养对清醒大鼠肾血流动力学的影响。
DOI：
10.1152/ajprenal.1986.250.3.f566
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Jamison,RL
中科院分区：
文献类型：
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作者：
GehrigJr,JJ;Jamison,RL;Baylis,C;Troy,JL;Brenner,BM;Jamison,RL
许多形式的饮食限制对有意识的大鼠的血流动力学影响尚未研究过。为了研究一种饮食限制方案（隔日喂养）对肾脏血流动力学的影响，我们对20只平均年龄30周、未麻醉、先前插管的雄性Sprague-Dawley大鼠进行了56项清除率研究。1组大鼠（n = 10）连续25周隔日给予标准饲料，饲喂后和禁食后进行研究；2组大鼠（n = 10）在同一时间段内自由饲喂，然后随机进行研究。1组饲后肾小球滤过率（GFR，清除率菊粉）和肾血流量[RBF，清除率PAH/（1 -红细胞压积）]分别比空腹增加23%和19% （GFR, 4.25 vs. 3.47 ml/min, P < 0.005； RBF, 22.2 vs. 18.6 ml/min, P < 0.025）。饲喂后，平均动脉压和血浆蛋白浓度不变，红细胞压积略有升高，钠排泄分数由0.23%上升至0.57%。组1的GFR和RBF平均比组2低31%，但当考虑体重因素时，差异就消失了。结果表明，隔日喂养引起健康清醒大鼠GFR和RBF的大幅波动，其机制不太可能仅涉及细胞外液容量的变化，并使GFR和RBF的平均值明显低于相同年龄的自由喂养大鼠。
The hemodynamic influences of many forms of dietary restriction have not been studied in the conscious rat. To examine the effect of one regimen of dietary restriction, alternate-day feeding, on renal hemodynamics, we performed 56 clearance studies in 20 unanesthetized, previously catheterized male Sprague-Dawley rats at mean age 30 wk. Group 1 rats (n = 10) were given standard chow only on alternate days for 25 wk and then studied after feeding and after fasting days, whereas group 2 rats (n = 10) were fed ad libitum during the same period and then studied randomly. In group 1, glomerular filtration rate (GFR, clearance of inulin) and renal blood flow [RBF, clearance of PAH/(1 - hematocrit)] increased 23 and 19%, respectively, after feeding days compared with fasting days (GFR, 4.25 vs. 3.47 ml/min, P less than 0.005; RBF, 22.2 vs. 18.6 ml/min, P less than 0.025). After feeding, mean arterial pressure and plasma protein concentration were unchanged, hematocrit increased slightly, and fractional excretion of sodium rose from 0.23 to 0.57%. Both GFR and RBF averaged 31% less in group 1 than in group 2 but, when factored by body weight, the differences were abolished. The results indicate that alternate-day feeding causes substantial oscillations in GFR and RBF in healthy awake rats through mechanisms unlikely to involve changes in extracellular fluid volume alone and limits GFR and RBF to average values markedly lower than those observed for larger ad libitum-fed rats of the same age.