Cells with osteoblastic phenotypes can be explanted from human gingiva and periodontal ligament
Cells with osteoblastic phenotypes can be explanted from human gingiva and periodontal ligament
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DOI：
10.1902/jop.1997.68.7.701
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发表时间：
1997-07-01
影响因子：
4.3
通讯作者：
Graves, DT
中科院分区：
文献类型：
--
作者：
Carnes, DL;Maeder, CL;Graves, DT
牙龈成纤维细胞的表型异质性已被报道，同样，牙周韧带(PDL)的细胞可以分离出不同表型的细胞，尽管有人认为牙龈细胞对硬组织的形成没有贡献，但理论上可能是在适当的刺激下，牙龈中的未成熟间充质细胞可以沿着成骨途径分化。未成熟间充质细胞在成骨诱导因子刺激下分化为成骨细胞已在肌肉中得到证实，我们进行了实验，以确定是否可以从人牙龈和人牙周韧带中鉴定出具有成骨细胞特征的细胞。这些组织中的某些细胞群具有较高的碱性磷酸酶基础活性，能在1，25(OH)(2)VitD(3)刺激下释放骨钙素，并形成矿化基质。因此，可以从具有表型标记的牙周组织和牙周组织中分离出具有成骨细胞特征的细胞，从牙周组织和牙周结缔组织中分离出具有成纤维细胞特征的其他细胞群。这些研究支持这样的概念，即牙龈组织可以产生沿成纤维细胞或成骨细胞途径分化的细胞。
CONSIDERABLE PHENOTYPIC HETEROGENEITY has been reported in gingival fibroblasts, Similarly, cells from the periodontal ligament (PDL) can be isolated with different phenotypes, Although it has been suggested that cells from the gingiva do not contribute to the formation of hard tissue, it is theoretically possible that under appropriate stimuli, immature mesenchymal cells in gingiva could differentiate along an osteoblastic pathway. Differentiation of immature mesenchymal cells into osteoblasts following stimulation with osteoinductive factors has been demonstrated in muscle, We undertook experiments to establish whether cells with osteoblastic characteristics could be identified from human gingiva as well as from human periodontal ligament. Some cell populations from each of these tissues were found to have high basal alkaline phosphatase activity, to release osteocalcin in response to 1,25(OH)(2)VitD(3), and to form a mineralized matrix. Thus, cells can be isolated from the gingiva and PDL that exhibit phenotypic markers, which taken together are characteristic of osteoblastic cells, Other cell populations derived from the PDL and gingival connective tissue were isolated that had fibroblastic characteristics. These studies support the concept that gingival tissue can give rise to cells which may differentiate along either a fibroblastic or an osteoblastic pathway.