Modifications of hydrophobicity, in vitro adherence and cellular aggregation of Streptococcus mutans by Helichrysum italicum extract
Modifications of hydrophobicity, in vitro adherence and cellular aggregation of Streptococcus mutans by Helichrysum italicum extract
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DOI：
10.1111/j.1472-765x.2004.01509.x
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发表时间：
2004-01-01
影响因子：
2.4
通讯作者：
Alonzo, V
中科院分区：
文献类型：
--
作者：
Nostro, A;Cannatelli, MA;Alonzo, V
目的：本研究的目的是研究亚致死浓度的意大利蜡菊提取物是否会影响变形链球菌的致龋特性。italicum（乙醇提取物）对口腔链球菌（Strep.变形链球菌ATCC 35668、链球菌唾液链球菌ATCC 13419和链球菌。sanguis ATCC 10556）及其对细胞表面疏水性、对玻璃表面的体外蔗糖依赖性粘附和链球菌的细胞聚集的影响。变异体结果表明，所有链球菌对乙醇提取物敏感，最小抑菌浓度（MIC）值为31.25-62.50 μ g/ml。H.意大利链球菌（7.81-31.25 μ g ml（-1））降低了链球菌的疏水性和对玻璃表面的粘附性（几乎90%）。变异体结论：葡聚糖T2000对H。意大利提取物对链球菌本研究的意义和影响：人们对使用天然化合物作为控制不良微生物的替代方法有相当大的兴趣。
Aims: The purpose of the present study was to examine whether sublethal concentrations of Helichrysum italicum extract could affect some of the cariogenic properties of Streptococcus mutans.Methods and Results: We studied the antibacterial activity of H. italicum (ethanolic extract) against oral streptococci (Strep. mutans ATCC 35668, Strep. salivarius ATCC 13419 and Strep. sanguis ATCC 10556) and its influence on cell-surface hydrophobicity, in vitro sucrose-dependent adherence to glass surface and cellular aggregation of Strep. mutans. The results indicate that all streptococci were susceptible to ethanolic extract with minimum inhibitory concentration (MIC) values of 31.25-62.50 mug ml(-1). Sub-MIC concentrations of H. italicum (7.81-31.25 mug ml(-1)) reduced the hydrophobicity and the adherence (almost 90%) to glass surface of Strep. mutans. The aggregation in the presence of dextran T2000 was also affected.Conclusion: The inhibitory activity of H. italicum extract on Strep. mutans is worthy of further study.Significance and Impact of the Study: There is considerable interest in the use of natural compounds as alternative methods to control undesirable micro-organisms.