Comparison of Bovine coronavirus-specific and Bovine respiratory syncytial virus-specific antibodies in serum versus milk samples detected by enzyme-linked immunosorbent assay
Comparison of Bovine coronavirus-specific and Bovine respiratory syncytial virus-specific antibodies in serum versus milk samples detected by enzyme-linked immunosorbent assay
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DOI：
10.1177/1040638713509377
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发表时间：
2014-01-01
影响因子：
1.5
通讯作者：
Fall, Nils
中科院分区：
文献类型：
--
作者：
Ohlson, Anna;Blanco-Penedo, Isabel;Fall, Nils
牛冠状病毒（BCV；β冠状病毒1）和牛呼吸道合胞病毒（BRSV）是全世界肉牛和奶牛肠道和呼吸道疾病的重要原因。间接酶联免疫吸附测定广泛用于检测血清抗体，用于畜群监测和患病率研究。在奶牛群中，牛奶比血清更容易收集。因此，为了研究血清和牛奶之间的测试一致性，对来自 27 个奶牛群的 105 头奶牛的血清和牛奶样本进行了平行分析，以确定是否存在针对 BCV 和 BRSV 的免疫球蛋白 G 抗体。 Bland-Altman 数据分析表明，两种病毒的血清和乳汁抗体滴度之间具有良好的一致性。结果表明牛奶样本足以监测 BCV 和 BRSV 抗体。
Bovine coronavirus (BCV; Betacoronavirus 1) and Bovine respiratory syncytial virus (BRSV) are significant causes of enteric and respiratory disease in beef and dairy cattle throughout the world. Indirect enzyme-linked immunosorbent assays are widely used to detect serum antibodies for herd monitoring and prevalence studies. In dairy herds, milk is more readily collected than serum. Hence, in order to investigate the test agreement between serum and milk, both serum and milk samples from 105 cows in 27 dairy herds were analyzed in parallel for presence of immunoglobulin G antibodies to BCV and BRSV. The Bland-Altman analyses of data demonstrated good agreement between serum and milk antibody titers for both viruses. The results indicate milk samples are sufficient for surveillance of antibodies to BCV and BRSV.