Alginate microparticles loaded with lipopolysaccharide subunit antigen for mucosal vaccination against Klebsiella pneumoniae
Alginate microparticles loaded with lipopolysaccharide subunit antigen for mucosal vaccination against Klebsiella pneumoniae
复制标题
DOI：
10.1016/j.biologicals.2015.02.001
复制
发表时间：
2015-05-01
期刊：
影响因子：
1.7
通讯作者：
Menon, M. D.
中科院分区：
文献类型：
--
作者：
Jain, R. R.;Mehta, M. R.;Menon, M. D.
肺炎克雷伯菌（K.pneumoniae）是人类医院感染的最常见原因之一。由于肺炎克雷伯菌感染是空气传播的类型，因此通过鼻腔和肺部途径进行粘膜疫苗接种来控制它可能是模拟自然感染的一种有前途的方法。亚单位疫苗等新疫苗比传统疫苗更安全，但免疫原性较低。因此，为了增强其免疫原性，需要开发有效且安全的佐剂和递送系统。已确定微粒是可用于粘膜递送疫苗的最有效的佐剂之一，它们通过提高抗原呈递细胞 (APC) 对封装抗原的摄取来实现这一点。脂多糖 (LPS) 是通过热苯酚提取方法从肺炎克雷伯菌中提取的抗原部分。采用乳液离子凝胶法制备负载脂多糖的海藻酸钠微粒。得到粒径小于5μm的微粒。 LPS负载微粒的负载效率范围为76%至82%。通过肌内、气管内和鼻内途径对瑞士白化小鼠进行游离 LPS 和封装 LPS 的体内免疫原性比较研究。研究表明，与游离 LPS 疫苗相比，通过气管内途径给药时，LPS 封装的微粒表现出更好的功效。 (C) 2015 年国际生物标准化联盟。由爱思唯尔有限公司出版。保留所有权利。
Klebsiella pneumoniae (K. pneumoniae) is one of the commonest causes of nosocomial infections in human beings. Since K. pneumoniae infections are air borne type, controlling it by mucosal vaccination through nasal and pulmonary route could be a promising approach in order to simulate the natural infection. New vaccines such as subunit vaccines are safer than traditional vaccines, but they are less immunogenic. Therefore to enhance their immunogenicity, there is a need to develop potent and safe adjuvants and delivery systems. It has been established that micro-particles are one of the most potent adjuvants available for mucosal delivery of vaccines and they do so by improving uptake of encapsulated antigen by antigen presenting cells (APCs).Lipopolysaccharide (LPS), the antigenic fraction was extracted from K. pneumoniae by hot phenol extraction method. LPS loaded sodium alginate microparticles were prepared by emulsion ionic gelation method. Microparticles with particle size less than 5 mu m were obtained. Loading efficiency of the LPS loaded microparticles ranged from 76 to 82 %. Comparative in vivo immunogenicity studies were carried for free LPS and encapsulated LPS, administered via intramuscular, intratracheal and intranasal routes in Swiss albino mice. The study revealed that LPS encapsulated microparticles exhibit greater efficacy when administered by intra-tracheal route as compared to free LPS vaccine. (C) 2015 The International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.