Simultaneous analysis of the time course for changes in core body temperature, activity, and nociception following systemic administration of interleukin-1beta in the rat.
Simultaneous analysis of the time course for changes in core body temperature, activity, and nociception following systemic administration of interleukin-1beta in the rat.
复制标题
同时分析大鼠全身给予白细胞介素-1β后核心体温、活动和伤害感受变化的时间过程。
DOI：
10.1016/j.brainres.2003.09.076
复制
发表时间：
2004
期刊：
影响因子：
2.9
通讯作者：
Heinricher,MaryM
中科院分区：
文献类型：
--
作者：
Morgan,MichaelM;Clayton,CecileaC;Heinricher,MaryM
伴随发烧的疼痛似乎至少部分是由白介素 1β (IL-1β) 等细胞因子的外周释放介导的。本研究的目的是确定 IL-1β 给药产生的伤害感受敏感性的变化是否与核心体温的变化暂时相关。实验1检查全身施用IL-1β（1、3、10和20μg/kg）后3小时内的伤害感受反应性。两种最高剂量的 IL-1β 在细胞因子施用后约 60 分钟开始产生温度下降。这种体温过低持续了 90 分钟，并与痛觉过敏有关。实验2检查了施用IL-1β（10μg/kg）后12小时内温度和伤害感受的变化。 IL-1β 给药后 3.25 至 6.5 小时内出现早期短暂的体温过低，随后出现明显的体温过高。这种迟发的发热伴有痛觉过敏。低温和高温阶段均与运动活动的减少相关。鉴于重复的伤害性测试可能会混淆温度和活动的评估，实验 3 检查了 IL-1β (10 μg/kg) 给药对留在笼中的大鼠的温度和活动的影响。温度的双相变化和活动的减少与实验 2 中报告的几乎相同，表明重复的伤害性测试不会混淆这些数据。本研究的结果表明，痛觉过敏发生了两个阶段，并且与全身施用 IL-1β 产生的体温调节改变的时期一致。
The aches and pains that accompany fever appear to be mediated, at least in part, by the peripheral release of cytokines such as interleukin-1β (IL-1β). The objective of this study was to determine, whether changes in nociceptive sensitivity produced by IL-1β administration are temporally linked to changes in core body temperature. Experiment 1 examined nociceptive responsiveness for a period of 3 h following systemic administration of IL-1β (1, 3, 10 and 20 μg/kg). The two highest doses of IL-1β produced a drop in temperature beginning approximately 60 min after cytokine administration. This hypothermia lasted 90 min and was associated with hyperalgesia. Experiment 2 examined changes in temperature and nociception for 12 h following administration of IL-1β (10 μg/kg). An early, short-lived hypothermia was followed by a significant hyperthermia from 3.25 to 6.5 h following IL-1β administration. This late-occurring fever was accompanied by hyperalgesia. Both the hypo- and hyperthermia phases were associated with a reduction in locomotor activity. Given that repeated nociceptive testing may confound assessment of temperature and activity, Experiment 3 examined the effects of IL-1β (10 μg/kg) administration on temperature and activity in rats that remained in their home cages. The biphasic change in temperature and the reduction in activity were nearly identical to that reported in Experiment 2, indicating that repeated nociceptive testing did not confound these data. The results of this study demonstrate that, two phases of hyperalgesia occur and coincide with the periods of altered thermoregulation produced by systemic administration of IL-1β.