Identification of novel suppressors for Mog1 implies its involvement in RNA metabolism, lipid metabolism and signal transduction.
Identification of novel suppressors for Mog1 implies its involvement in RNA metabolism, lipid metabolism and signal transduction.
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DOI：
10.1016/j.gene.2007.06.012
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发表时间：
2007-10
期刊：
影响因子：
3.5
通讯作者：
M. Oki;Li Ma;Yonggang Wang;Akira Hatanaka;Chie Miyazato;K. Tatebayashi;H. Nishitani;H. Uchida;T. Nishimoto
中科院分区：
文献类型：
--
作者：
M. Oki;Li Ma;Yonggang Wang;Akira Hatanaka;Chie Miyazato;K. Tatebayashi;H. Nishitani;H. Uchida;T. Nishimoto
Mog1从酵母到哺乳动物都是保守的，但其功能尚不清楚。我们分离出的酵母基因挽救了酿酒酵母Scmog1Δ和pombe spmogts的温度敏感死亡。Scmog1Δ被磷脂n-甲基转移酶Opi3p、酵母ran同源物Gsp1p和RanGDP结合蛋白Ntf2p拯救。另一方面，spmog1ts被Cid13拯救，Cid13是编码核糖核苷酸还原酶亚基的suc22+mRNA特异性的多聚(a)聚合酶，Ssp1是参与应激反应途径的蛋白激酶，Crp79是mRNA输出所需的，此外还有Spi1， S. pombe ran同源物和Nxt2， S. pombe同源物Ntf2p。与这些抑制因子的鉴定一致，缺乏ScMog1p使核膜上的Opi3p脱位，所有的spmog1s都显示出mRNA的核积累。SpMog1与Nxt2和Cid13共析出。
Mog1 is conserved from yeast to mammal, but its function is obscure. We isolated yeast genes that rescued a temperature-sensitive death of S. cerevisiae Scmog1Δ, and of S. pombe Spmog1ts. Scmog1Δ was rescued by Opi3p, a phospholipid N-methyltransferase, in addition to S. cerevisiae Ran-homologue Gsp1p, and a RanGDP binding protein Ntf2p. On the other hand, Spmog1tswas rescued by Cid13 that is a poly (A) polymerase specific for suc22+mRNA encoding a subunit of ribonucleotide reductase, Ssp1 that is a protein kinase involved in stress response pathway, and Crp79 that is required for mRNA export, in addition to Spi1, S. pombe Ran-homologue, and Nxt2, S. pombe homologue of Ntf2p. Consistent with the identification of those suppressors, lack of ScMog1p dislocates Opi3p from the nuclear membrane and all of Spmog1tsshowed the nuclear accumulation of mRNA. Furthermore, SpMog1 was co-precipitated with Nxt2 and Cid13.