Microscopic and submicroscopic structure of the synapsis in the ventral ganglion of the acoustic nerve.
Microscopic and submicroscopic structure of the synapsis in the ventral ganglion of the acoustic nerve.
复制标题
听神经腹侧神经节突触的显微和亚显微结构。
DOI：
10.1016/0014-4827(54)90172-x
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发表时间：
1954
影响因子：
3.7
通讯作者：
E. De Robertis
中科院分区：
文献类型：
--
作者：
C. Estable;M. Reissig;E. De Robertis
本工作选择听神经腹侧神经节为研究神经元间突触的显微镜和亚显微镜材料，比较了锇酸固定的超薄切片与银染厚片（30 μ）和薄片（0.5 ~ 1 μ）的电镜观察。显微镜下可见各种各样的突触终末，包括端足、棒形终末和Held球，几乎完全包围着细胞体和树突，电镜下可见典型的棒形终末和端足，与细胞表面紧密接触。在突触连接处，突触前和突触后的原生质被高电子密度的膜分开，这是围绕终末的鞘的延续。这种鞘具有与神经纤维的髓鞘相同的外观和电子密度。在突触连接处，膜的厚度在2000和320埃之间变化。一些较薄的细胞膜呈双层结构，突触前原生质电子密度很低，可见少量原纤维和线粒体形态的卵形颗粒。在某些情况下，轴突膜厚约100 μ m，突触后原生质更致密，电子密度更高。在突触附近可见线粒体和较小的颗粒。
The ventral ganglion of the acoustic nerve was selected for a microscopic and submicroscopic study of the interneuronal synapsis.In this work the electron microscope observations made on ultrathin sections of osmic acid fixed material were compared with silver-stained thick (30 μ) and thin sections (0.5 to 1 μ) of the same material. The microscopic observations show a wide variety of synaptic terminals including end-feet, club-endings and Held's bulbs surrounding almost completely the body of the cell and dendrites.The electron microscope observation shows typical club-like endings and end-feet in intimate contact with the cell surface. At the level of the synaptic junction the pre- and postsynaptic protoplasm are separated by a membrane of high electron density which is a continuation of the sheath which surrounds the terminal. This sheath has the same appearance and electron density as the myelin sheath of the nerve fibers. At the synaptic junction the membrane varies in thickness between 2000 and 320 Å. Some of the thinner membranes have a double lamellar appearance.The presynaptic protoplasm has a very low electron density and shows a few protofibrils and ovoidal granules of mitochondrial aspect. In some cases there are indications of an axon membrane about 100 Å thick.The postsynaptic protoplasm is more compact and of higher electron density. Mitochondria and smaller granules are observed in the neighborhood of the synapsis.