Stress history and pubertal development interact to shape hypothalamic-pituitary-adrenal axis plasticity
Stress history and pubertal development interact to shape hypothalamic-pituitary-adrenal axis plasticity
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DOI：
10.1210/en.2005-1432
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发表时间：
2006-04-01
期刊：
影响因子：
4.8
通讯作者：
McEwen, BS
中科院分区：
文献类型：
--
作者：
Romeo, RD;Bellani, R;McEwen, BS
激素应激反应的幅度和持续时间在新生儿发育和衰老过程中以及先前的应激经历中都发生了巨大变化。然而，令人惊讶的是，很少有人知道青春期成熟和经验与压力相互作用，影响下丘脑-垂体-肾上腺轴的反应。由于青春期是一个神经发育脆弱和机会的时期，可能对压力特别敏感，因此必须更充分地了解这些相互作用。因此，我们研究了激素和神经反应在青春期前（28天的年龄）和成年（77天的年龄）雄性大鼠暴露于急性（30分钟）或慢性（30分钟/天，7天）约束应激后。我们在这里报告，急性应激后，青春期前的男性表现出显着延长激素应激反应（如促肾上腺皮质激素和总的和游离皮质酮）与成年人相比。与此相反，慢性压力后，青春期前的男性表现出较高的反应后，立即紧张，但更快地恢复到基线，与成年人相比。此外，我们表明，这种差异的应激反应与差异的神经元激活下丘脑室旁核，FOS免疫组化测定。使用三重标记免疫荧光组织化学，我们发现，一个更大比例的CRH，但不是精氨酸加压素，细胞被激活的精氨酸加压素在青春期前的动物相比，急性和慢性应激。这些数据表明，下丘脑-垂体-肾上腺神经内分泌轴的经验依赖性可塑性显着影响青春期成熟。
Both the magnitude and the duration of the hormonal stress response change dramatically during neonatal development and aging as well as with prior experience with a stressor. However, surprisingly little is known with regard to how pubertal maturation and experience with stress interact to affect hypothalamic-pituitary-adrenal axis responsiveness. Because adolescence is a period of neurodevelopmental vulnerabilities and opportunities that may be especially sensitive to stress, it is imperative to more fully understand these interactions. Thus, we examined hormonal and neural responses in prepubertal (28d of age) and adult (77d of age) male rats after exposure to acute (30 min) or more chronic (30 min/d for 7 d) restraint stress. We report here that after acute stress, prepubertal males exhibited a significantly prolonged hormonal stress response (e.g. ACTH and total and free corticosterone) compared with adults. In contrast, after chronic stress, prepubertal males exhibited a higher response immediately after the stressor, but a faster return to baseline, compared with adults. Additionally, we demonstrate that this differential stress reactivity is associated with differential neuronal activation in the paraventricular nucleus of the hypothalamus, as measured by FOS immunohistochemistry. Using triple-label immunofluorescence histochemistry, we found that a larger proportion of CRH, but not arginine vasopressin, cells are activated in the arginine vasopressin in response to both acute and chronic stress in prepubertal animals compared with adults. These data indicate that experiencedependent plasticity of the hypothalamic-pituitary-adrenal neuroendocrine axis is significantly influenced by pubertal maturation.