Infection with HIV type 1 group m non-B subtypes in individuals living in New York City
Infection with HIV type 1 group m non-B subtypes in individuals living in New York City
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DOI：
10.1097/00126334-200407010-00011
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发表时间：
2004-07-01
影响因子：
3.6
通讯作者：
Nyambi, PN
中科院分区：
文献类型：
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作者：
Achkar, JM;Burda, ST;Nyambi, PN
目的：了解纽约市居民感染HIV-1 M组非B亚型的情况。设计：从1999年10月至2003年4月，从纽约市3家门诊就诊的HIV-1阳性患者中筛选出感染HIV-1非B亚型的高危人群。根据异源双链迁移率分析或测序和系统发育分析的结果确定感染HIV-1的M亚型。结果：97名研究对象进入研究。在97名受试者中，91名(94%)是根据从非欧洲国家移民而来的，而6名(6%)是美国本土公民。在检测的97份血浆样本中，有53份(55%)被成功确定了亚型。2份血浆标本的亚型无法分类。HIV-1感染分为M亚型：A(n=4；7%)、B(n=12；22%)、C(n=8；15%)、F(n=2；4%)、CRF01_AE样(n=7；13%)、CRF02AG样(n=19；34%)、亚型间重组G(Gag)/A(Env)(n=1；2%)和无法分类的病毒(n=2)；结论：这项研究揭示了多种HIV-1 M组亚型的感染，主要是在纽约市的移民人口中，以及几个非B亚型是如何被引入美国的。
Objective: To document infection with HIV type 1 (HIV-1) group M non-B subtypes in individuals living in New York City.Design: From October 1999 through April 2003, HIV-1-seropositive individuals were selected from 3 clinics in New York City based on having risk factors for infection with HIV-1 non-B subtypes.Methods: HIV-1 RNA was extracted from plasma samples, and partial gag, pol, or env genes were amplified by PCR analysis. The infecting HIV-1 group M subtype was determined based on results of either heteroduplex mobility assay or sequencing and phylogenetic analysis.Results: Ninety-seven subjects were enrolled in the study. Of the 97 subjects, 91 (94%) were selected based on having emigrated from a non-European country, while 6 (6%) were native United States citizens. Subtypes were successfully determined in 53 (55%) of the 97 plasma samples tested. The subtypes in 2 plasma samples were unclassifiable. HIV-1 infections were classified as those due to the following group M subtypes: A (n = 4; 7%), B (n = 12; 22%), C (n = 8; 15%), F (n = 2; 4%), CRF01_AE-like (n = 7; 13%), CRF02_AG-like (n = 19; 34%), an intersubtype recombinant form G(gag)/A(env) (n = 1; 2%), and unclassifiable viruses (n = 2; 4%).Conclusion: This study reveals infection with a broad variety of HIV-1 group M subtypes mostly in the immigrant population of New York City as well as how several non-B subtypes are being introduced into the United States.